福建农业学报2017，Vol.32Issue(9)：963-968,6.DOI:10.19303/j.issn.1008-0384.2017.09.009
玉竹愈伤组织诱导及其细胞悬浮培养体系的建立
Induction and Suspension Culture of Calluses from Polygonatum odoratum
摘要
Abstract
Effects of hormone,carbon source,and pH on induction,as well as that of hormone on proliferation of callus tissues from Polygonatum odoratum were studied.Rhizomes and leaves of the plants were used as the explants in the experiment.In addition,the suspension culture for propagating the callus cells was optimized.The results showed that the optimal medium for the induction of rhizome and leaf calluses was MS+ 1.0 mg 6-BA · L-1 +0.5 mg NAA · L-1 +30％ sucrose at pH 5.6.The optimal hormone combination for the callus proliferation was 1.5 mg 6-BA · L-1 and 0.6 mg NAA · L-1.The rates of callus induction and proliferation of the rhizomes were significantly higher than those of the leaves.For the suspension culture on rhizome callus cells,the optimum inoculum rate was 60 g · L-1,and the hormone combination of 1.5 mg 6-BA · L-1 +0.6 mg NAA · L-1.The growth and polysaccharide content of the cells in the suspension exhibited an S-shape curve with an optimal subculture cycle of 16 d.The polysaccharide content in the cells peaked after 14 d cultivation,while the pH of the medium decreased initially followed by an increase,and leveled off subsequently.关键词
玉竹愈伤组织/诱导/增殖/细胞悬浮培养Key words
Polygonatum odoratum/callus/induction/proliferation/cell suspension culture分类
农业科技引用本文复制引用
王争艳,王霞,王欢..玉竹愈伤组织诱导及其细胞悬浮培养体系的建立[J].福建农业学报,2017,32(9):963-968,6.基金项目
吉林农业科技学院青年基金项目[吉农院合字(2014)第214号 （2014）
吉林农业科技学院大学生创新项目[吉农院合字(2015)第103号] （2015）
