福建农业学报2017，Vol.32Issue(9)：1006-1011,6.DOI:10.19303/j.issn.1008-0384.2017.09.016
发酵床陈化垫料纤维素降解真菌的分离及其堆肥效果研究
Isolation and Composting Efficiency of Cellulose-degrading Fungus from Litter Fermentation Bed in Pig Pan
摘要
Abstract
A fungus,M6,that displayed a powerful ability to degrade cellulose was isolated from the waste fermentation beds at pig farms using the CMC-Na plate and Congo red staining methods.ITS sequence of the isolated strain was blasted with those from NCBI database,and the phylogenetic tree constructed to arrive at a preliminary identification for the fungus as Penicillium oxalate.Subsequently,an optimized enzyme-producing fermentation process on a solid medium inoculated with the isolated fungi was established.It included the application of bran and CMC-Na as the carbon source,soybean meal as the nitrogen source,pH 6.0 at the start,10％ inoculation rate,and fermentation at 30℃ for 96 h.The carboxymethyl cellulase activity of the material increased 2.31 times due to the optimization that reached 1 446 U · g-1.In a composting experiment on the fermentation beds inoculated with M6,EM or control,M6 and EM raised the bed temperature to greater than 50℃ on the 3rd day after start of fermentation,and maintained at the level for 10 d and 6 d,respectively.The cellulose degradation rate was 41.1％ with M6,38.8％ with EM,and 14.8％ with control.It suggested that,among the 3 tested fungi,M6 was most efficient on the waste treatment for pig farms.关键词
草酸青霉/固态发酵/羧甲基纤维素酶/发酵床Key words
Penicillium oxalate/solid fermentation/carboxymethyl cellulose/pig-litter fermentation bed分类
农业科技引用本文复制引用
杜东霞,许隽,杜宇,尹红梅,刘惠知..发酵床陈化垫料纤维素降解真菌的分离及其堆肥效果研究[J].福建农业学报,2017,32(9):1006-1011,6.基金项目
湖南省自然科学基金项目(2015JJ3084) （2015JJ3084）
湖南省科技重点研发计划项目(2016NK2208) （2016NK2208）
