口腔疾病防治2018，Vol.26Issue(2)：83-89,7.DOI:10.12016/j.issn.2096-1456.2018.02.003
口腔鳞状细胞癌组织中环状RNA的差异表达谱分析
Differential expression of circular RNA in oral squamous cell carcinoma
摘要
Abstract
Objective To analyze circular RNA (circRNA) expression profiles in oral squamous cell carcinoma (OSCC) and its clinical significance. Methods The expression of circRNA was detected with circRNA microarray as-say in three samples of OSCC tumor and matched adjacent tissues. Quantitative real-time PCR (RT-qPCR) was used to verify the expression of circRNA in 45 pair OSCC tissues and normal adjacent tissues. The relationship between the ex-pression of circRNA and the clinicopathological characteristics of OSCC was analyzed. circRNAs/miRNAs interaction were predicted using Arraystar' s home-made miRNA target prediction software. Results 155 circRNAs were differen-tially expressed between the OSCC tissues and matched adjacent tissues, of which 45 circRNAs were up-regulated and 110 circRNAs were down-regulated in OSCC tissues (fold changes ≥1.5 and P<0.05). In the selected three circRNAs that were most significantly upregulated or downregulated in OSCC, the RT-qPCR results showed that hsa_circ_0001874, hsa_circ_0001971 and has_circ_0067934 were increased, while hsa_circ_0000520, hsa_circ_0023944 and hsa_circ_0000140 were decreased in OSCC tissues versus normal adjacent tissues (P<0.001). The results were general-ly consistent with the microarray data. Among the circRNA expression profiles in OSCC, the up-regulation of hsa_circ_0001874 was the highest and the expression of hsa_circ_0001874 was significantly correlated with TNM stage and tu-mor grade. The result of Arraystar ' s home-made miRNA target prediction software indicated that miR-103a-3p, miR-107, miR-593-5p, miR-661 and miR-662 may be potential target genes of hsa_circ_0001874. Conclusion The differ-entially expressed circRNAs in OSCC tissues and normal adjacent tissues were identified, and these dysregulated cir-cRNAs and their potential target gents may play important roles in the development of OSCC.关键词
环状RNA/口腔/鳞状细胞/癌/癌旁组织/基因芯片/RT-qPCR/靶基因Key words
Circular RNA/Oral/Squamous cell/Carcinoma/Matched adjacent tissues/Microarray/Quantitative real-time PCR/Target genes分类
医药卫生引用本文复制引用
赵思语,欧阳少波,王军,黄自坤,罗清,廖岚..口腔鳞状细胞癌组织中环状RNA的差异表达谱分析[J].口腔疾病防治,2018,26(2):83-89,7.基金项目
国家自然科学基金项目(81660444) （81660444）
江西省自然科学基金项目(20171BAB205050) （20171BAB205050）
江西省重点研发计划项目(20161BBG70149) （20161BBG70149）
江西省卫生和计生委科技项目(20175296) （20175296）
