南方农业学报2018，Vol.49Issue(1)：142-147,6.DOI:10.3969/j.issn.2095-1191.2018.01.23
雷州黑鸭FSHβ基因多态性与产蛋性能的关联分析
FSHβ gene polymorphism and correlation between it and egg production performance in Leizhou black duck
摘要
Abstract
[Objective]The genetic variation of follicle stimulating hormone β subunit(FSHβ)gene in Leizhou black duck population was clarified to provide molecular markers for breeding of Leizhou black duck population.[Method]The polymorphisms of exon2 and intron 1(int1)of gene FSHβ in 200 Leizhou black ducks were detected by PCR-SSCP,and the polymorphic sites(SNPs)were found and sequenced.The correlation between different genotypes and egg production traits of Leizhou black ducks was analyzed.[Result]A single nucleotide mutation site(A→T)was found at g.1103451A>T (int1)of gene FSHβ in Leizhou black duck.There were three genotypes(AA,AT and TT).The corresponding genotype frequencies were 0.538,0.359 and 0.103 respectively.The frequency of T allele(0.718)was higher than that of allele A (0.282),and g.1103451A>T locus was in Hardy-Weinberg equilibrium(P>0.05)in Leizhou black duck population.Age at first egg of TT genotype was the earliest,followed by AT genotype,which were 5.48 and 4.00 d earlier than AA geno-type respectively,and the difference reached significant level(P<0.05,the same below).In terms of weight at first egg and egg production at 300 day-age,TT and AT individuals were also significantly higher than those of AA individuals,and TT individuals were the highest(1299.31 g/feather and 134.37 eggs respectively).The rest of the egg production traits in each genotype were not significantly different between the individuals(P>0.05).[Conclusion]There is a SNP lo-cus(g.1103451A>T)in int1 of Leizhou black duck gene FSHβ,which has significant correlation with its egg production performance.It mainly promotes early egg laying and growth,hence it may be assisted-selection molecular markers for Leizhou black duck breeding.关键词
雷州黑鸭/FSHβ基因/SNP位点/产蛋性能/关联分析Key words
Leizhou black duck/gene FSHβ/single nucleotide polymorphisms(SNP)site/egg production/correla-tion analysis分类
农业科技引用本文复制引用
张少伟,崔红艳,苏瑛..雷州黑鸭FSHβ基因多态性与产蛋性能的关联分析[J].南方农业学报,2018,49(1):142-147,6.基金项目
广东省科技计划项目(2014A020208123) （2014A020208123）
广东省公益研究与能力建设专项项目(2017A020208066) （2017A020208066）
湛江市现代农业重点项目(2015A03003) （2015A03003）
广东海洋大学研究生优秀学位论文培育项目(201610) （201610）
