广西医科大学学报2017，Vol.34Issue(12)：1681-1685,5.DOI:10.16190/j.ckni.45-1211/r.2017.12.001
Construction,identification and biological feature study of human hepatocellular carcinoma cells stably expressing HBeAg
Construction, identification and biological feature study of human hepatocellular carcinoma cells stably expressing HBeAg
摘要
Abstract
Objective:To construct a HepG2 cell line which stably expressing Hepatitis B e antigen (HBeAg) and investigate mthe effects of HBeAg on the proliferation,migration and invasion of HepG2 cells.Methods:The lentivirus carrying HBeAg gene was constructed and packaged.HepG2 cells were infected with the lentivirus and screened with puromycin to obtain HepG2 cells which stably expressing HBeAg (HepG2-HBeAg cells).The expression levels of HBeAg mRNA and protein were detected by RT-qPCR and Western blot,respectively.The content of HBeAg secretion in cell supernatant in both HepG2-HBeAg cells and control cells (HepG2-NC cells and HepG2 cells) were detected by IFMA assay.Furthermore,CCK-8 proliferation assay,colony formation assay and transwell migration and invasion assays were conducted to compare the abilities of cell proliferation,migration and invasion,respectively.Results:The expression of HBeAg in the HepG2-HBeAg cell was significantly higher than those in HepG2 cells and HepG2-NC cells.Secreted HBeAg in the supernatant of HepG2-HBeAg cells was 26.33 ± 2.13 PEIU/mL but was undetectable in supernatant of control cells.The proliferation,migration and invasion were all significantly lower in HepG2-HBeAg cells compared to control cells (P＜0.01).Conclusion:A HepG2 cell line which stably expressing HBeAg was constructed successfully,and the over-expression of HBeAg could attenuate the proliferation,migration and invasion of hepatoeellular carcinoma cells.关键词
HBeAg/hepatocellular carcinoma cells/proliferation/migration/invasionKey words
HBeAg/hepatocellular carcinoma cells/proliferation/migration/invasion分类
医药卫生引用本文复制引用
Yuxuan Li,Shan Huang,Kezhi Li,Guobin Wu,Hao Tian,Yinnong Zhao..Construction,identification and biological feature study of human hepatocellular carcinoma cells stably expressing HBeAg[J].广西医科大学学报,2017,34(12):1681-1685,5.基金项目
This work was supported by the National Natural Science Foundation of China (No.81360315) and Guangxi Medical and Health Technology Development and Application Project (No.S201629). （No.81360315）
