华北农学报2017,Vol.32Issue(z1):7-11,5.DOI:10.7668/hbnxb.2017.S1.002
小麦抗白粉病基因pm30种质改良及鉴定
Enhancement and Molecular Identification of Wheat Germplasm Resources with Resistance Gene Pm30 to Powdery Mildew
摘要
Abstract
Wheat powdery mildew is one of the main diseases threaten wheat production in China even all over the world. Screening and development disease-resistant varieties is a safe and effective way for prevention and con-trol of wheat powdery mildew. The purpose of this study is to introduce powdery mildew resistance gene Pm30 into the wheat varieties Jizi 356 with advanced comprehensive agronomic characters and high yield developed by au-thors. One crosses,Jizi 356/Synthetics He-48 were made. Twelve lines with field resistance to powdery mildew and excellent agronomic characters were developed after six years of offspring screening. Then,seven lines were used for identifying resistance gene Pm30 using Xgwm159 SSR marker. Chinese spring and Pm30 were used as a contrast. The results showed that two new lines 14YF650 and 14YF675 could amplify the 430,460,500 bp polymorphic frag-ments. It indicated the two lines carrying Pm30 powdery mildew resistance gene derived from wild emmer. Those lines with big spikes,resistant to lodging and excellent agronomic characters could be used directly as parents for wheat powdery mildew resistance breeding.关键词
普通小麦/野生二粒小麦/抗病基因/白粉病/Pm30/分子标记Key words
Common wheat/Wild emmer/Resistance gene/Powdery mildew/Pm30 gene/Molecular marker分类
生物科学引用本文复制引用
兰素缺,李光威,孟雅宁,李芳,张业伦,李杏普..小麦抗白粉病基因pm30种质改良及鉴定[J].华北农学报,2017,32(z1):7-11,5.基金项目
小麦种质资源发掘与创新利用(2013BAD01B02-11)小麦抗白粉病种质资源创新(11220112D) (2013BAD01B02-11)
小麦种质资源表型精准鉴定与优质、抗病等种质创新(2016YFD0100102-5) (2016YFD0100102-5)
冬小麦种质资源与育种技术创新(16226320D) (16226320D)