华北农学报2017,Vol.32Issue(z1):12-16,5.DOI:10.7668/hbnxb.2017.S1.003
高粱丝黑穗病抗性基因SRAP标记的初步分析
Preliminary Analysis on SRAP Markers for Head Smut Resistance in Sorghum
摘要
Abstract
For screening SRAP markers associated to head smut resistance in sorghum,and to select and identi-fy resistant sorghum in the laboratory,achieving the real molecular marker-assisted breeding,a sensitive sorghum va-riety Sanchisan,and a resistant variety Tx622B were selected,as research materials. Their morphological and agro-nomical characters were observed,and the polymorphic SRAP markers were screened. The results showed that there were significant differences in the field characters between Sanchisan and Tx622B,the plant height,stem diameter, culm height,numbers of stem nodes above ground,panical length,numbers of panicle nodes,panicle kernel weight and 1 000 kernel weight of Sanchisan were higher than Tx622B,its primary branch number was higher than Tx622B,in plot was less than Tx622B;The difference of yield traits between the two varieties reached a very signif-icant level. And 16 pairs of polymorphic primers were identified between the two varieties,which can be further used for identifying head smut resistance-associated genes in sorghum. It provided basis for selecting and breeding resistant sorghum varieties to head smut.关键词
高粱/丝黑穗病/SRAP标记/抗性基因Key words
Sorghum/Head smut/SRAP marker/Resistance gene分类
农业科技引用本文复制引用
段永红,张旭,崔福柱,司浩浩,孙毅,张福耀..高粱丝黑穗病抗性基因SRAP标记的初步分析[J].华北农学报,2017,32(z1):12-16,5.基金项目
高粱遗传与种质创新山西省重点实验室开放课题(2015K-03) (2015K-03)
山西省自然科学基金项目(201701D121105) (201701D121105)
山西省财政支农项目(2015TGSF-10) (2015TGSF-10)
国家公益性行业(农业)科研专项(201503121-07) (农业)