河南农业大学学报2017，Vol.51Issue(5)：640-646,661,8.
利福平对毛泡桐长链非编码RNA表达谱变化的影响
Changes of expression of long non-coding RNAs in Paulownia tomentosa treated with rifampin
摘要
Abstract
To elucidate the regulation of lncRNAs in the growth and development of Paulownia,the high-throughput sequencing technology was used to explore the expression of long non-coding RNA and its target genes after being treated with rifampin in Paulownia tomentosa.A total of 129 differentially expressed long non-coding RNAs and 289 corresponding target genes were identified.GO analysis showed that these target genes involved in 9 categories,and most of them involved in cellular process,metabolic process,single-organism process and other classifications.KEGG pathway analysis indicated that these differentially expressed target genes were involved in plant hormone signal transduction and secondary metabolic biosynthesis.To confirm the expression of Paulownia lncRNAs and their target genes,qRT-PCR analysis was performed to verify the results of the RNA-Seq.Six lncRNAs and their corresponding target genes were selected randomly from the PT and PTL-100 libraries for quantitative PCR.The result suggested that the RNA-seq dataset was dependable.关键词
毛泡桐/利福平处理/长链非编码RNAKey words
Paulownia tomentosa/rifampin treatment/long non-coding RNA分类
农业科技引用本文复制引用
李永生,李冰冰,曹亚兵,王哲,董焱鹏,邓敏捷,赵振利,范国强..利福平对毛泡桐长链非编码RNA表达谱变化的影响[J].河南农业大学学报,2017,51(5):640-646,661,8.基金项目
国家公益性林业行业科研专项基金项目(201004002) （201004002）
