河南农业大学学报2017，Vol.51Issue(5)：657-661,5.
麦长管蚜重要基因的RNA干扰
RNA interference of important genes in Sitobion avenae
摘要
Abstract
In this study RNA interference (RNAi) technology was applied to analyze the effect of RNAi silencing of tweleve important genes of wheat aphids by feeding aphids with double stranded RNA (dsRNA) of the target genes.The results showed that the mortality of wheat aphids significantly increased after RNAi silencing of water channel protein gene (Wsa) and ATP synthase gene (vAd1-78);whereas glucose dehydrogenase encoding gene (GlD-17) RNAi led to a significantly reduced mortality.Quantitative analysis of gene expression level during 5 days after RNAi showed that the expression level of gene Wsa and vAd1-78 was decreased by 74.8％ and 44.6％,respectively.Whereas the expression level of gene GlD-17 was increased by up to 209％.Variance on mortality of wheat aphids was in accordance with gene expression level changes after gene silencing by RNAi.Among the twelve targeted genes,RNA interference of Wsa led to most significant gene silence effect,increasing mortality to 37.5％ and down-regulating gene expression level to 74.8％.Thus water channel protein gene (Wsa)could be used as a candidate targeted gene of RNA interference for further development of wheat varieties of aphids resistance.关键词
麦长管蚜/RNA干扰(RNAi)/dsRNA饲喂/基因沉默效应Key words
Sitobion avenae/RNA interference (RNAi)/double stranded RNA feeding/gene silencing effect分类
农业科技引用本文复制引用
白福强,谢纳,夏晴,董振杰,麦艳娜,刘文轩..麦长管蚜重要基因的RNA干扰[J].河南农业大学学报,2017,51(5):657-661,5.基金项目
国家自然科学基金项目(31571658) （31571658）
河南省重大科技专项(161100110400) （161100110400）
省部共建小麦玉米作物学国家重点实验室开放课题基金项目(39990022) （39990022）
