河南农业大学学报2017，Vol.51Issue(5)：699-704,6.
硝普钠对藠头鳞茎贮藏期间碳氮代谢及关键酶活性的影响
Effects of sodium nitroprussiate on the metabolites and key enzyme activities of carbon-nitrogen metabolism in rokkyo bulbs during storage
摘要
Abstract
In this study,Kaiyuan sweet rokkyo bulbs were used as material to determine the sugar content,enzyme activity in carbon and nitrogen metabolism and so on in different concentration of sodium nitroprusside.The results showed that during storage,the contents of starch and total nitrogen decreased,but the contents of soluble sugar,reducing sugar,sucrose and protein increased gradually;free amino acids content increased firstly and then decreased;sodium nitroprusside treatment increased the extent of increasing or decreasing.The activities of amylase,nitrate reductase (NR) and glutamine synthetase (GS) first increased and then decreased;sucrose phosphate synthase (SPS) and sucrose synthase (SS) activity decreased;glutamate synthase (GOGAT) activity increased;sodium nitroprusside treatment increased the activities of these enzymes,and delayed the decline.In addition,sodium nitroprusside treatment also increased the total carbon content,effected the ratio of carbon and nitrogen,effectively inhibited the growth of shoots during storage,and eased decay and fresh weight loss.Generally,sodium nitroprusside with O.50 mmol · L-1 is more effective to promote the conversion of starch and sugar accumulation,delay nitrogen decomposition,promote the accumulation of protein,and improve the appearance quality of rokkyo bulbs.关键词
藠头/硝普钠/贮藏/碳氮代谢/酶活性Key words
rokkyo/sodium nitroprusside/storage/carbon and nitrogen metabolism/enzyme activity分类
轻工纺织引用本文复制引用
张永福,郭丽红,任禛,靳松,姚丽媛..硝普钠对藠头鳞茎贮藏期间碳氮代谢及关键酶活性的影响[J].河南农业大学学报,2017,51(5):699-704,6.基金项目
国家自然科学基金项目(31660559) （31660559）
昆明学院科学研究项目(XJZZ1604) （XJZZ1604）
云南省高校特色生物资源开发与利用重点实验室开放基金项目(GXKJ201615). （GXKJ201615）
