中医学报2018，Vol.33Issue(1)：5-9,14,6.DOI:10.16368/j.issn.1674-8999.2018.01.002
五味消毒饮抑制人皮肤鳞状细胞癌A431细胞增殖及促凋亡作用研究
Effect of Wuwei Xiaodu drink on proliferation inhibition and promoting apoptosis of A431
摘要
Abstract
Objective:To study the effect of Wuwei Xiaodu drink on proliferation inhibition and promoting apoptosis of skin squamous cancer cell (A431).Methods:The human epidermal carcinoma A431 cell line was selected as the experimental material,and the flavonoid disinfection drink was used to treat the human skin squamous cell carcinoma A431 cells cultured in vitro.The blank control group and the study group were set as contml group,being cultured for 24 h,48 h and 72 h respectively.The cell inhibitory rate was measured by MTT assay.The morphology of A431 cells was observed.The expression of Bcl-2 and Bax in human skin squamous cell carcinoma A431 cells was detected by immunohistochemistry.The cell apoptosis was analyzed with flow cytometry.Results:The inhibitory rates of proliferation of A431 cells cultured in human skin squamous cell carcinoma 24 h,48 h and 72 h respectively of the study group were significantly higher than that of the blank control group respectively,and the inhibitory rate of cell proliferation significantly increased with the prolongation of time (P ＜ 0.05).Morphologically,the A431 cells of the human skin squamous cell carcinoma in the blank control group showed polygons,irregular shape,strong refraction,uniform distribution,adherent growth and good cell growth state.The human skin squamous cell carcinoma cells A431 manifested in poor growth performance,cell volume reduction,shrinkage round,the surrounding cells reduce the adhesion,the gap between cells expanded,with the incubation time,the cells continue to fall off or die and the number of cells was significantly reduced.Flow cytometry showed that the apoptosis rate of A431 cells in the study group was significanlty higher than that in the blank control group (P ＜0.05).The positive rate of Bax cells in the study group was significantly higher than that in the blank control group (P ＜ 0.05).Conclusion:Wuwei Xiaodu Drink can reduce the expression of Bcl-2,promote the expression of Bax,inhibit the proliferation of human skin squamous cell carcinoma A431 cells,promote the apoptosis of A431 ceils and control or inhibit the occurrence and development of human skin squamous cell carcinoma.关键词
五味消毒饮/人皮肤鳞状细胞癌A431细胞/肿瘤/细胞增殖/细胞凋亡/Bcl-2/Bax/中医药治疗Key words
Wuwei Xiaodu Drink/human skin squamous cell carcinoma A431 cells/tumor/cell proliferation/apoptosis/Bcl-2/Bax/Chinese medicine treatment分类
医药卫生引用本文复制引用
谭文英..五味消毒饮抑制人皮肤鳞状细胞癌A431细胞增殖及促凋亡作用研究[J].中医学报,2018,33(1):5-9,14,6.基金项目
国家自然科学基金资助项目(81260311) （81260311）
