中医学报2018，Vol.33Issue(1)：99-105,7.DOI:10.16368/j.issn.1674-8999.2018.01.026
补肺益肾方对慢性阻塞性肺疾病模型大鼠肺泡表面活性蛋白A、D及肺泡结构的影响
Effects of Bufei Yishen Formula on Surfactant-associated Proteins A,D and Alveolar Structure of COPD Rats
摘要
Abstract
Objective:To explore the effect of Bufei Yishen Formula on Surfactant-associated Proteins A,D and Alveolar Structure of rats with chronic obstructive pulmonary disease (COPD).Methods:36 rats were randomized into control group,model group,Bufei Yishen high-dose group,Bufei Yishen middle-dose group,Bufei Yishen low-dose group and aminophyline group.COPD rats were induced by cigarette-smoke and bacterial exposures,and were administrated with normal saline,Bufei Yishen Formula,with 7.4 g,3.7 g and 1.85 g · (kg· d)-1 in each dose,and the aminophyline group was given aminophylline,with 54 mg · (kg· d)-1 in each dose.The treatment lasted for 12 weeks and the rats were sacrificed.Lung pathology,ultrastructure of alveolar type Ⅱ epithelial cell,SP-A,D mRNA and protein expression in lung tissue were measured.Results:Lung pathology:There is no marked pulmonary impairment was observed in the control rats,while rats in model group showed severe pathological such as alveolar destruction,alveolar cavity expansion,bronchiole stenosis,inflammatory cells infiltration and mucosal hyperplasia.The pathological changes were alleviated in the treatment groups at different degrees,especially in Bufei Yishen middle-dose groups.The ultrastructure of alveolar type Ⅱ epithelial cell:In Control group,abundant mitochondria and lamellar bodies arranged in concentric circles or in parallel were present in the cytoplasm of type Ⅱ alveoli epithelial cells.In Model group,the limited and small mitochondria,degranulated and cavitated lamellar bodies were observed in type Ⅱ alve61i epithelial cells.In Bufei Yishen high-dose group,the rich mitochondria and clear mitochondrial cristae,as well as few lamellar bodies with complete structure were observed in type Ⅱ alveoli epithelial cells.In Bufei Yishen middle-dose group,the rich mitochondria with clear mitochondrial cristae,abundant,complete lamellar bodies,and complete marginal villi were observed in type Ⅱ alveoli epithelial cells.In Bufei Yishen low-dose and aminophyline groups,the limited mitochondria and lamellar bodies were observed.QPCR:Compared with that of the control group,SP-A,SP-D mRNA were decreased significantly in model group (P≤0.01).Compared with that of the model group,SP-A,SP-D mR-NA in Bufei Yishen high-dose group,middle-dose group,and aminophyline group increased significantly (P≤0.01).Compared with that of the Bufei Yishen high-dose group and middle-dose group,SP-A and SP-D mRNA in Bufei Yishen low-dose and aminophyline group decreased significantly (P≤0.01).Laser scanning confocal microscope:Compared with that of the control group,the SP-A,SP-D positive cells were decreased significantly in model group (P≤0.01);Compared with that of the model group,SP-A,SP-D positive cells in Bufei Yishen high-dose,middle-dose,and aminophyline groups increased significantly (P ≤0.01).Compared with that of the Bufei Yishen high-dose group,SP-A,SP-D positive cells in Bufei Yishen middle-dose increased (P≤0.05 or P0.01).Compared with that of the Bufei Yishen middle-dose groups,SP-A,SP-D positive cells in Bufei Yishen low-dose group and aminophyline group decreased significantly (P≤0.01).Conclusion:Bufei Yishen Formular can improve the lamellar body of alveolar type Ⅱ epithelial cell,regulate the synthesis and secretion of surfactant-associated proteins and maintain alveolar structure stability,in which the effect of Bufei Yishen Formula middle-dose is the most significant.关键词
补肺益肾方/慢性阻塞性肺疾病/肺泡表面活性蛋白/肺泡结构/大鼠Key words
Bufei Yishen Formula/chronic obstructive pulmonary disease/surfactant-associated proteins/alveolar structure/rat分类
医药卫生引用本文复制引用
田燕歌,张蓝熙,冀会鸽,董浩然,刘学芳,李建生,冯素香,毛静,董玉琼,李君子,卞晴晴..补肺益肾方对慢性阻塞性肺疾病模型大鼠肺泡表面活性蛋白A、D及肺泡结构的影响[J].中医学报,2018,33(1):99-105,7.基金项目
国家自然科学基金项目(81403367) （81403367）
河南省教育厅科学技术研究重点项目(15A360027,15A360022) （15A360027,15A360022）
