化工进展2018，Vol.37Issue(2)：525-532,8.DOI:10.16085/j.issn.1000-6613.2017-0859
小球藻在内光源气升式反应器中的培养工艺优化
Optimization of culture technology of Chlorella vulgaris in airlift reactors with internal light source
摘要
Abstract
To explore the optimal cultivation conditions of Chlorella vulagris in the airlift photo-bioreactor,a custom-designed 50 L airlift reactor with an internal light source was developed,and the influence of light wavelength,intensity of light source,light and dark cycles and supply of carbon dioxide on the growth of Chlorella vulagris were investigated in this study.Single factor experiments were firstly conducted with algae cell density as the investigation index.Based on that,the response surface methodology was further adapted to optimize the process conditions.The optimal conditions were as follows: red-blue lights as light source,9615 lux of light intensity,17.5h:6.5h of light-dark cycles and 30L/h of carbon dioxide ventilation.Under the optimal conditions,three verification experiments were performed.After 15 days cultivation,the harvested cell density of Chlorella vulagris was 5.48×107cells/mL,which was close to the results predicted,and the dry weight was 1.21 g/L,increased by 157%.These results can provide an important reference for the application of internal light source airlift reactor in microalgae culture.关键词
小球藻/内光源气升式反应器/响应面法/藻细胞密度Key words
Chlorella vulagris/air-lift reactor with internal light source/response surface methodology/algae cell density分类
能源科技引用本文复制引用
张芬芬,马晓建,方书起,白净,常春..小球藻在内光源气升式反应器中的培养工艺优化[J].化工进展,2018,37(2):525-532,8.基金项目
河南省自然科学基金(162300410247)及河南省基础与前沿技术项目(162300410007). （162300410247）
