渔业科学进展2018，Vol.39Issue(1)：97-106,10.DOI:10.11758/yykxjz.20161128001
三疣梭子蟹F型ATP酶β亚基(F-ATPaseβ)基因的克隆、组织表达及在家系近交中的变化
cDNA Cloning and Expression Analysis of F-ATPaseβ Subunit Gene in Portunus trituberculatus and Its Variation in Family Inbreeding
摘要
Abstract
A full-length cDNA sequence of F-ATPase β subunit gene from Portunus trituberculatus (ptF-ATPaseβ)was cloned by rapid amplification of cDNA ends(RACE).The sequence of ptF-ATPaseβ was 1965 bp, containing a 571 bp 5' UTR, 341 bp 3' UTR, and a 1053 bp open reading frame (ORF) that encodes 350 amino acids polypeptides. The isoelectric point (pI) was 4.86 and the molecular mass was 37.9 kDa.The amino acid sequence analysis demonstrated that ptF-ATPaseβ has an F1-ATPaseβ domain, an AAA domain, and an ATP-synt-ab-C domain. Homology and phylogenetic analysis revealed that the amino acid sequence of ptF-ATPaseβ shared a high similarity (89%) with Penaeus monodon and Litopenaeus vannamei. ptF-ATPaseβ mRNA level was detected in all tested tissues including the hepatopancreas,muscle,heart,gill,stomach,intestine,testis,and ovary.The ptF-ATPaseβ had the highest level in the hepatopancreas and heart, and the lowest expression in the intestine. With the increase of inbreeding coefficient, ptF-ATPaseβ expression decreased significantly in the hepatopancreas and heart (P<0.05). The ATP synthase activity in the heart began to fall from F6generation and was significantly lower than F0generation (P<0.05), but there was no significant change in the hepatopancreas. The results illustrate that inbreeding gradually reduces the expression of ptF-ATPaseβ and the ATP synthase activity in P.trituberculatus.关键词
三疣梭子蟹/F-ATPaseβ/基因克隆/表达分析/近交Key words
Portunus trituberculatus/ptF-ATPaseβ/Gene cloning/Expression analysis/Inbreeding分类
农业科技引用本文复制引用
王竹青,任宪云,高保全,刘萍,张小辉,张杰,于旋..三疣梭子蟹F型ATP酶β亚基(F-ATPaseβ)基因的克隆、组织表达及在家系近交中的变化[J].渔业科学进展,2018,39(1):97-106,10.基金项目
国家自然科学基金面上项目(41576147)、泰山领军人才工程高效生态农业创新类计划项目(LJNY2015002)和中央级公益性科研院所基本科研业务费专项资金资助项目(20603022015016)共同资助[This work was supported by National Natural Science Foundation of China(41576147),the Program of Shandong Leading Talent(LJNY2015002),and the Special Scientific Research Funds for Central Non-Profit Institutes(20603022015016)]. （41576147）
