生物质化学工程2018，Vol.52Issue(1)：10-16,7.DOI:10.3969/j.issn.1673-5854.2018.01.002
金银忍冬提取物的挥发性成分及抑菌活性分析
Analysis of Volatile Components and Antibacterial Activity of Lonicera maackii(Rupr.) Maxim. Extracts
摘要
Abstract
Active substance of Lonicera maackii(Rupr.) Maxim. fresh leaves and fresh flowers were respectively extracted by Soxhlet extraction method with hexane as solvent. The volatile components were analyzed by gas chromatography-mass spectrometry(GC-MS),and their antibacterial activities were analyzed by antibacterial activity experiment. The results showed that the highest GC content compound was (-)-isolongifolol(22.63%),the second was 2,7,10-trimethyl-dodecane(10.48 %) in the fresh leaves extracts of Lonicera maackii. The highest GC content compound was 2,7,10-trimethyl-dodecane(14.16 %), and the second was the dibutyl phthalate(9.60 %) in the fresh flowers extracts of Lonicera maackii. When the mass concentration of fresh leaves extracts of Lonicera maackii was 0.19 g/mL,the inhibitory effect on Staphylococcus aureus was the highest with the antibacterial circle diameter of 13.81±1.48 mm. Lonicera maackii(Rupr.) Maxim. fresh flowers extract had good inhibition effect on Escherichia coli in the mass concentration of 0.027 5-0.22 g/mL range. According to the half inhibitory concentration of Lonicera maackii(Rupr.) Maxim. extract on three kinds of test strains,the results showed that Lonicera maackii(Rupr.) Maxim. fresh leaves extract and its flower extract of three kinds of experimental bacteria followed the inhibitory sequence of Staphylococcus aureus> Escherichia coli > Bacillus subtilis.关键词
金银忍冬提取物/GC-MS/抑菌活性Key words
Lonicera maackii extracts/gas chromatography-mass spectrometry(GC-MS)/antibacterial activity分类
化学化工引用本文复制引用
高欣妍,王海英,刘志明,段晓玲,冯馨慧..金银忍冬提取物的挥发性成分及抑菌活性分析[J].生物质化学工程,2018,52(1):10-16,7.基金项目
黑龙江省自然科学基金资助项目(C201411) （C201411）
技术开发项目企业横向课题(07043215003) （07043215003）
