临床与实验病理学杂志2017，Vol.33Issue(10)：1063-1069,7.DOI:10.13315/j.cnki.cjcep.2017.10.001
结直肠腺癌组织中USP10的表达及生物信息学分析
Expression of USP10 in colorectal adenocarcinoma and analysis of bioinformatics
摘要
Abstract
Purpose To investigate the expression and significance of USP10 protein and mRNA in normal colorectal mucosa and colorectal adenocarcinoma,and to analyze the cause of the disorder.Methods 99 cases of colorectal adenocarcinoma and 83 cases of normal intestinal mucosa tissue were selected.Using tissue microarray and immunohistochemistry the expression of USP10 protein was detected,and the relationship was analyzed between USP10 protein and clinical pathological parameters or prognosis survival time.The expression of USP10 mRNA was analyzed by GEO datesets.Some miRNAs that down-regulate the expression of USP10 protein were screened by bioinformatics methods.The expression of USP10 protein and miR-149 in colorectal cancer cell lines were detected by Western blot and real-time quantitative PCR.Results The positive rate of USP10 protein in normal intestinal mucosa tissues was 71.08％(59/83),which was significantly higher than that in colorectal adenocarcinoma tissues (53.54％,53/99,P =0.015).No correlation were proved between USP10 protein expression and clinical pathological parameters or survival time (P ＞ 0.05).The expression level of USP10 mRNA in colorectal adenocarcinoma was 1.07 ～ 1.45 times that were higher than that of normal intestinal mucosa,which showed that the down-regulation of USP10 protein was at the post-transcriptional level.The program predicted a putative highly-conserved binding site in the USP10 mRNA 3'UTR for miR-149 which was up regulated in colorectal adenocarcinoma tissues.In addition,the expression of miR-149 was negatively correlated with the expression of USP10 protein in colorectal cancer cell lines.Conclusion The down-regulation of USP10 protein which occurs at the post-transcriptional level is closely related to the pathogenesis of colorectal adenocarcinoma.The high expression of miR-149 may be one of the factors that negatively regulate the expression of USP10 protein.关键词
结直肠肿瘤/结直肠腺癌/USP10/miR-149/生物信息学Key words
colorectal neoplasms/colorectal adenocarcinoma/USP10/miR-149/bioinformatics分类
医药卫生引用本文复制引用
曾智,阎红琳,陈龙艳,田山,黄亚冰,古丽娟,熊晓星,袁静萍..结直肠腺癌组织中USP10的表达及生物信息学分析[J].临床与实验病理学杂志,2017,33(10):1063-1069,7.基金项目
国家自然科学基金(81602535)、湖北省自然科学基金面上项目(2016CFB249) （81602535）
