临床与实验病理学杂志2017，Vol.33Issue(10)：1070-1073,4.DOI:10.13315/j.cnki.cjcep.2017.10.002
胃癌中RBBP4和BCL-2的表达及意义
Expression of RBBP4 and BCL-2 in gastric carcinoma and their clinicopathological significance
摘要
Abstract
Purpose To investigate the expression and significance of RBBP4 and BCL-2 in gastric carcinoma and to explore the relationship between their expression and clinicopathologic characteristics.Methods Immunohistochemistry was used to detect RBBP4 and BCL-2 proteins' expression in the 72 tumor tissues and para-cancer tissues.The association of RBBP4 expression with the clinicopathologic features and the expression correlation between RBBP4 and BCL-2 were analyzed.Real-time PCR was used to detect the expression of RBBP4 mRNA in the 20 tumor tissues and corresponding para-cancer tissues.Results Immunohistochemical results showed that 40 cases of cancer tissues exhibited RBBP4 positive expression,with a positive rate of 55.6％,and 36 cases showed BCL-2 positive expression (50％ positive rate),which were significantly higher than that in para-cancer mucosa (13.9％,16.7％).The over-expression of RBBP4 and BCL-2 was correlated with tumor differentiation,invasive depth and lymphatic metastasis in gastric carcinoma (P＜ 0.05).The expression of RBBP4 was positively correlated with BCL-2 (r =0.559,P ＜ 0.05).Real-time PCR results showed that the relative expression levels of RBBP4 mRNA in gastric carcinoma tissues and para-cancer tissues were 9.988 ± 1.948 and 1.458 ± 0.489 respectively,and the expression in gastric carcinoma was significantly up-regulated as compared with para-cancer tissues.Conclusions The expression of RBBP4 is up-regulated in gastric carcinoma,which may be involved in the development,invasion and metastasis of gastric carcinoma.RBBP4 might become a new diagnostic and prognostic marker for gastric carcinoma.关键词
胃肿瘤/RBBP4/BCL-2/免疫组织化学/实时荧光定量PCRKey words
gastric neoplasms/RBBP4/BCL-2/immunohistochemistry/Real-time PCR分类
医药卫生引用本文复制引用
曹嬿,范松龙,张智弘..胃癌中RBBP4和BCL-2的表达及意义[J].临床与实验病理学杂志,2017,33(10):1070-1073,4.基金项目
国家自然科学基金(81773109)、江苏省自然科学基金(BK20151582) （81773109）
