临床与实验病理学杂志2017，Vol.33Issue(10)：1086-1091,6.DOI:10.13315/j.cnki.cjcep.2017.10.006
肺癌组织及细胞中MED27的表达及意义
Expression of MED27 in lung cancer tissues and cells and its significance
摘要
Abstract
Purpose To investigate the expression level of MED27 in lung cancer tissue samples and lung cancer cell lines and to further study the biological function of MED27 in lung cancer cells.Methods Immunohistochemistry and Western blot were used to detect MED27 expression in 70 lung cancer tissues and 5 different lung cancer cell lines,and the correlation between MED27 expression and gender,age as well as PTNM was also analyzed.The silence sequence of MED27 was designed by the siRNA technique.Western blot was used to detect the silence efficiency of MED27.The proliferation,migration and invasion ability of cells were assessed by CCK-8 assay,Scratch assay and Transwell assay after the MED27 was knocked down.Western blot was used to detect the expression of protein involved in the cell proliferation,migration and invasion.Results The results of immunohistochemistry and Western blot showed that MED27 expression was higher in lung cancer tissues and cells (P ＜ 0.05).The expression of MED27 was positively correlated with lymph node metastasis (x2 =9.438,P =0.002,P ＜ 0.05).However,it was not related with gender,age,tumor size and distant metastasis (P ＞ 0.05).The knockdown of MED27 by MED27 specific siRNA could inhibit the proliferation,migration and invasion of H460 cells (P ＜ 0.05).The expression of MMP-2 and MMP-9 involved in the cell migration that were significantly inhibited in H460 cells transfected by MED27 siRNA,and the expression of E-cadherin,related with cell invasion was also decreased,while E-cadherin negative regulatory protein Snail was increased.Conclusion MED27 is highly expressed in lung cancer tissues and cells and high expression of MED27 predicts poor prognosis in lung cancer patients.The knockdown of MED27 inhibits the proliferation,migration and invasion ability of lung cancer cells.All of the above results suggest that MED27 is expected to be a candidate target of lung cancer gene therapy.关键词
肺肿瘤/中介因子复合体27/迁移/侵袭Key words
lung neoplasms/MED27/migration/invasion分类
医药卫生引用本文复制引用
朱晓静,许鹏飞,曹彦,谢开鹏,唐冉冉..肺癌组织及细胞中MED27的表达及意义[J].临床与实验病理学杂志,2017,33(10):1086-1091,6.基金项目
国家自然科学青年基金(81702831、81402139) （81702831、81402139）
