中国临床保健杂志2018，Vol.21Issue(1)：88-91,4.DOI:10.3969/J.issn.1672-6790.2018.01.025
基质金属蛋白酶-2在脑胶质瘤细胞中的表达及其分子探针体外磁共振成像的检测
Expression of MMP2 in brain glioma cells and detection of magnetic resonance imaging in vitro by molecular probes
摘要
Abstract
Objective Observation of matrix metalloproteinase -2(MMP2)expression in the glioma cells,and study its molecular probes magnetic resonance imaging ability in vitro.Method The expression level of MMP2 was de-tected by Western blotting in various brain glioma cell line;USPIO-PEG-MMP2Ab was synthetized by conjugating USPIO-PEG with MMP2Ab.MTT colorimetric assay was used to assess the cytotoxicity of USPIO-PEG-MMP2Ab.The specific binding ability of USPIO-PEG and USPIO-PEG-MMP2Ab with MMP2 highly expressed glioma cells was detected by Prussian blue staining and MR imaging in vitro.Result All the tumor cells were positive for MMP2,there were sig-nificant differences compared with astrocytes;The synthetic molecular probe USPIO-PEG-MMP2Ab has no cytotoxicity in the range of concentration,and has the ability to bind specifically to C6 glioma cells.Prussian blue staining showed that at the same time and concentration conditions,USPIO-PEG-MMP2Ab group showed much more intracellular blue parti-cles than USPIO-PEG group.Magnetic resonance imaging showed that the SI value of the group USPIO-PEG-MMP2Ab cells under the same concentration condition decreased significantly,and compared with the USPIO-PEG group cells,the difference was statistically significant.Conclusion MMP2 is highly expressed in glioma cells, and the USPIO-PEG-MMP2Ab molecular probe has high activity and safety.It is targeted against glioma cell lines and lays the foundation in vivo experiments.关键词
神经胶质瘤/基质金属蛋白酶2/磁共振成像/分子探针Key words
Glioma/Matrix metalloproteinase 2/Magnetic resonance imaging/Molecular probes分类
医药卫生引用本文复制引用
孙艳,沈静,单煜恒,钟士江..基质金属蛋白酶-2在脑胶质瘤细胞中的表达及其分子探针体外磁共振成像的检测[J].中国临床保健杂志,2018,21(1):88-91,4.基金项目
中国人民武装警察部队后勤学院附属医院种子基金(FYM201535) （FYM201535）
天津市卫生行业重点攻关项目(16KG136) （16KG136）
