肿瘤预防与治疗2017，Vol.30Issue(5)：330-336,7.DOI:10.3969/j.issn.1674-0904.2017.05.002
中性粒细胞对肺癌血管生成的影响及机制研究
The Role and Mechanism of Neutrophil in Lung Cancer Angiogenesis
摘要
Abstract
Objective:To detect the relationship between neutrophil activation and lung cancer angiogenesis.Methods:Neutrophils,differentiated from HL-60 by DMSO,were activated by LPS and co-culture with lung cancer cell.Low expression of PARP-1 neutrophil line was constructed by lentivirus infection.Angiogenesis assay was detected after stimulation with neutrophils activation products.Realtime-PCR,Western blot and gelatin zymography were used to detect the expression of the main angiogenesis factors including VEGF and MMP-9.Results:The neutrophil activation products significantly enhanced the angiogenesis ability of lung cancer.Western Blot assay suggested that PARP-1kd neutrophils were successfully constructed and the ability of low expression of PARP-1 neutrophils on the promotion of lung cancer angiogenesis was weakened.The results of gelatin zymography and Western Blot assay showed that the activity and the expression of MMP-9 protein was negatively correlated with PARP-1 expression.Realtime-PCR results showed that the expression of VEGF and MMP-9 in PARP-1kd neutrophils was significantly decreased.Conclusion:Neutrophils promote the angiogenesis of lung cancer by regulating the expression of MMP-9 and VEGF through PARP-1 gene.关键词
肺癌/中性粒细胞/PARP-1/血管生成Key words
Lung cancer/Neutrophil/PARP-1/Angiogenesis分类
医药卫生引用本文复制引用
韩露露,周晓琬,冯倩,吕艳芳,李慧忠,王刚,郑骏年..中性粒细胞对肺癌血管生成的影响及机制研究[J].肿瘤预防与治疗,2017,30(5):330-336,7.基金项目
国家自然科学基金(编号:81400055),江苏省自然科学基金(编号:BK20140242) （编号:81400055）
