肿瘤预防与治疗2017，Vol.30Issue(5)：337-342,6.DOI:10.3969/j.issn.1674-0904.2017.05.003
顺铂通过抑制PI3K/AKT/mTOR信号通路诱导子宫内膜癌Ishikawa细胞自噬
Cisplatin Induces Endometrial Carcinoma Ishikawa Cell Autophagy by Inhibiting PI3K / AKT / mTOR Signaling Pathway
摘要
Abstract
Objective:To investigate the effect of cisplatin on autophagy of Ishikawa cells and the role of PI3K /AKT/mTOR signal transduction pathway in endometrial carcinoma.Methods:TEM (transmission electron microscope) was used to observe the formation of autophagic vacuoles,fluorescence microscope was used to observe the fluorescence aggregation of green fluorescent protein and microtubule-associated protein 1 light chain 3 fusionprotein Ⅱ (LC3 Ⅱ),so as to detect whether cisplatin could induce autophagy in Ishikawa endometrial cancer cells.Western blot was performed to detect expressions of PI3K,AKT and mTOR proteins in mTOR pathway.Results:Cisplatin induced the autophagy of Ishikawa cells.Compared with 20 μg/mL-12 h cisplatin group,more autophagosomes were obversed in the 20 μg/mL-24 h group(P ＜ 0.05).LC3Ⅱ levels gradually increased in 20 μg/mL-12 h group and 20 μg/mL-24 h group in a time-dependent manner comparing with that in the control group.The expression levels of phosphorylated AKT1,phosphorylated mTOR and PI3K p85 decreased significantly over time.The expressions of autophagosome and LC3 Ⅱ protein in co-culture group treated with IGF-1 were lower than those in pure cisplatin group,but higher than those of the control group.Conclusion:Cisplatin could induce the autophagy in Ishikawa endometrial cancer cells and PI3K/Akt/mTOR signaling pathway may be involved in this process.关键词
顺铂/子宫内膜癌/自噬/PI3K/AKT/mTOR信号通路Key words
Cisplatin/Endometrial cancer/Autophagy/PI3K/AKT/mTOR signaling pathway分类
医药卫生引用本文复制引用
林琼燕,生秀杰,刘娟,熊汉真,王沂峰..顺铂通过抑制PI3K/AKT/mTOR信号通路诱导子宫内膜癌Ishikawa细胞自噬[J].肿瘤预防与治疗,2017,30(5):337-342,6.基金项目
广州市卫生局一般引导项目资助项目(编号:20151A010106) （编号:20151A010106）
