上海针灸杂志2018，Vol.37Issue(2)：234-238,5.DOI:10.13460/j.issn.1005-0957.2018.02.0234
针刺内关、水沟对脑缺血再灌注大鼠大脑皮层Caspase-3、PARP-1表达的影响
Effect of Acupuncture at Points Neiguan(PC6) and Shuigou(GV26) on Caspase-3 and PARP-1 Expressions in the Cerebral Cortex in Rats with Cerebral Ischemia-reperfusion
摘要
Abstract
Objective To investigate the inhibiting effect of acupuncture at points Neiguan(PC6) and Shuigou(GV26) on caspase-3, and PARP-1 mRNA and protein expressions in the cerebral cortex in rats with cerebral ischemia-reperfusion. Method Forty male SD rats were randomized to normal, sham operation, model and acupuncture groups. A model of middle cerebral artery infarction was made by modified Longa thread occlusion. Neurological function was scored using a 6-grade 5-point Bederson's scale. Caspase-3, and PARP-1 mRNA and protein expressions in rat cerebral cortex were determined using real-time PCR and Western Blot at 24 hrs after cerebral ischemia. Result Neurological deficit was reduced in the acupuncture group of rats with cerebral ischemia-reperfusion compared with the model group (P<0.01). Acupuncture could down-regulate caspase-3, and PARP-1 mRNA and protein expressions and there was a statistically significant difference compared with the model group (P<0.05, P<0.01; P<0.05, P<0.05). Conclusion Acupuncture at points Neiguan and Shuigou can down-regulate caspase-3, and PARP-1 mRNA and protein expressions in the cerebral cortex in rats with cerebral ischemia-reperfusion.关键词
针刺/细胞凋亡/再灌注损伤/Real-timePCR/WesternBlot/Caspase-3/PARP-1/大鼠Key words
Acupuncture/Apoptosis/Reperfusion injury/Real-time PC/Western Blot/Caspase-3/PARP-1/Rats分类
医药卫生引用本文复制引用
程莹莹,倪光夏,狄忠..针刺内关、水沟对脑缺血再灌注大鼠大脑皮层Caspase-3、PARP-1表达的影响[J].上海针灸杂志,2018,37(2):234-238,5.基金项目
国家自然科学基金面上项目(81674067) （81674067）
浙江省自然科学基金一般项目(LY17H270009) （LY17H270009）
