色谱2018，Vol.36Issue(2)：143-149,7.DOI:10.3724/SP.J.1123.2017.10018
分散微固相萃取-超高效液相色谱-高分辨质谱法测定葡萄酒和啤酒中多菌灵和噻菌灵
Determination of carbendazim and thiabendazole in wine and beer by ultra high performance liquid chromatography-high resolution mass spectrometry coupled with dispersive micro solid-phase extraction
摘要
Abstract
A rapid method was established for the determination of carbendazim and thiaben-dazole in wine and beer by ultra high performance liquid chromatography-Q Orbitrap high reso-lution mass spectrometry and dispersive micro solid-phase extraction(DMSPE)based on a strong cation exchange adsorbent(PCX). For the pretreatment method,the amount of PCX, the volume percentage of acetonitrile,the volume percentage of ammonium hydroxide and the volume of eluent were optimized. The analytes were separated on a BEH C18column(50 mm× 2.1 mm,1.7 μm)and detected using targeted single ion monitoring-data dependent tandem mass spectrometry(tSIM-ddMS2)scan mode. The method showed a good linearity within a cer-tain concentration ranges with correlation coefficients R2≥0.999 9. The detection limits of car-bendazim and thiabendazole in wine and beer were 0.02 and 0.01 μg/L,and the quantification limits were 0.06 and 0.03 μg/L. The average recoveries of carbendazim and thiabendazole at the spiked levels of 0.1,1.0 and 100 μg/L were in the ranges of 95.6%-110.2% and 87.5%-102.8%,with intra-day precision(RSDr)ranges from 1.8%-5.2% and 1.3%-4.8%,and inter-day precision(RSDR)ranges from 4.3%-8.7% and 4.8%-9.4%. The method is rapid,simple and sen-sitive for the simultaneous determination of carbendazim and thiabendazole in wine and beer.关键词
分散微固相萃取/超高效液相色谱/高分辨质谱/多菌灵/噻菌灵/葡萄酒/啤酒Key words
dispersive micro solid-phase extraction(DMSPE)/ultra high performance liquid chromatography(UHPLC)/high resolution mass spectrometry(HRMS)/carbendazim/thia-bendazole/wine/beer分类
化学化工引用本文复制引用
高洁,陈达炜,赵云峰..分散微固相萃取-超高效液相色谱-高分辨质谱法测定葡萄酒和啤酒中多菌灵和噻菌灵[J].色谱,2018,36(2):143-149,7.基金项目
国家食品安全风险评估中心高层次人才队伍建设523项目.China Food Safety Talent Competency Development Initiative:China National Center for Food Safety Risk Assessment(CFSA)523 Program. （CFSA）
