山西农业大学学报（自然科学版）2018，Vol.38Issue(1)：21-24,54,5.DOI:10.13842/j.cnki.issn1671-8151.201707022
谷子SibZIP8转录因子基因功能鉴定
Gene function analysis of foxtail millet gene SibZIP8
摘要
Abstract
[Objective]The bZIP transcription factor gene family,plays crucial roles in various plant developmental and physio-logical processes,it can inhibit or activate the downstream gene transcription in the process of response to stress in plants, thus enhancing the ability of plant to adapt to the environment.This study provided experimental data for further study of the function of SibZIP8 gene.[Methods]In the previous study,a genome-wide analysis of SibZIP genes and expression profi-ling under stress was performed and SibZIP8 was identified as a putative drought-resistance gene.In this study,in order to further identify the function of SibZIP8 gene,over-expression of SibZIP8 was applied in Arabidopsis and the resistance phe-notype of transgenic plants was analyzed.[Results]The results showed that wild germination of Arabidopsis thaliana and transgenic Arabidopsis were not found under 1.5 μmol·L -1ABA treatment,significant difference was not found between wild type Arabidopsis thaliana and transgenic Arabidopsis at 300 mmol·L -1mannitol,under NaCl conditions,wild-type Ar-abidopsis did not germinate,while transgenic Arabidopsis sprouted.[Conclusion]SibZIP8 transgenic plants were more resist-ant to salt than wild type plant.In conclusion,our study should make a great contribution in genetic improvement of millet cultivation with stress resistance.关键词
谷子/bZIP转录因子/逆境胁迫/功能鉴定/拟南芥Key words
Foxtail millet/bZIP transcription factor/Abiotic stress/Functional characterization/A rabidopsis thaliana分类
农业科技引用本文复制引用
刘盼,张艾英,张莉..谷子SibZIP8转录因子基因功能鉴定[J].山西农业大学学报（自然科学版）,2018,38(1):21-24,54,5.基金项目
国家自然科学基金项目(31501323) （31501323）
山西省回国留学人员科研资助项目(2016-07) （2016-07）
