中国农业科技导报2018，Vol.20Issue(2)：129-138,10.DOI:10.13304/j.nykjdb.2017.0198
油酸诱导斑马鱼肝脏细胞脂滴形成与降解模型的建立
Establishment of Lipid Droplets Formation and Degradation Models Induced by Oleic Acid in Zebrafish Hepatocyte
摘要
Abstract
In order to establish zebrafish hepatocytes lipid droplets formation and degradation models,this experiment cultured the zebrafish hepatocytes by different concentrations of oleic acid (OA) to find out the optimal OA concentration for the subsequent study by measuring cell viability and apoptosis with Alarmablue assay and flow cytometry,respectively.Subsequently,the cells were cultured with media added the optimal OA concentration for 72 h.At the end of OA incubation,the cells were cultured with media removed OA.During the OA-supplement and -free periods,the lipid accumulation status was detected by oil red staining and TAG quantify.The cells with different sizes of lipid droplets were observed by BODIPY/HOECHST staining.The percentage of cells with supersized lipid droplets reached the maximun at 72 h.Compared with 0 h,the mRNA level of diacylglycerol acyhransferase 2 (DGAT2) was significantly upregulated from 6 h to 72 h after 100 μmol/L OA exposure.The mRNA level of Adipose differentiation-related protein(ADRP)was significantly upregulated at 24 h after removing OA.The mRNA level of fat storage-inducing transmembrane protein gene (FIT1) was significantly upregulated at 6 h after OA exposure.During the whole OA treatment and the OA removing period,the mRNA level of CIDEb,member of CIDE protein family,was significant upregulated.In conclusion,these results demonstrated that the zebrafish hepatocytes models of lipid droplets formation and degradation induced by OA could mimic the process of lipid droplets formation and degradation in fish liver.The models have provided a model basis for further studies on lipid droplets protein.关键词
油酸/脂滴/肝细胞/斑马鱼Key words
oleic acid/lipid droplet/hepatocyte/zebrafish分类
农业科技引用本文复制引用
丁倩雯,张震,冉超,杜震宇,周志刚..油酸诱导斑马鱼肝脏细胞脂滴形成与降解模型的建立[J].中国农业科技导报,2018,20(2):129-138,10.基金项目
国家973计划项目(2014CB138600) （2014CB138600）
北京市现代农业产业技术体系项目(SCGWZJ20151104-4)资助. （SCGWZJ20151104-4）
