中国动物传染病学报2018，Vol.26Issue(1)：7-12,6.
PRRSV感染对猪肺泡巨噬细胞IFITM3基因转录的影响
EFFECT OF PRRSV INFECTION ON IFITM3 TRANSCRIPTION IN PORCINE ALVEOLAR MACROPHAGES
摘要
Abstract
As an antivirus protein, interferon inducible transmembrane protein 3 (IFITM3) inhibits a broad spectrum of virus infections. In this study, the specific primers were designed based on the genes of IFITM3 and GAPDH to detect IFITM3 expression in PRRSV-infected porcine alveolar macrophages (PAMs). The recombinant plasmids containing the target genes were constructed as a standard control and real time SYBR Green I RT-PCR method was developed for detection of IFITM3 expression. The results showed that the detection limit of this method was 102copies/μL with good linear relationship, specificity, sensitivity and repeatability. Moreover, the variation coefficient of the method was less than 3%. We used this method to detect the IFITM3 mRNA levels in PRRSV-infected PAMs. The results revealed that the IFITM3 mRNA levels showed no significant difference at the early stage of infection but increased significantly at the late stage of infection in PRRSV-infected PAMs as compared with mock-infected PAMs, demonstrating that the changes of IFITM3 mRNA levels in PRRSV-infected PAMs might be related to PRRSV infection as well as immune evasion.关键词
猪干扰素诱导跨膜蛋白3/猪繁殖与呼吸综合征病毒/SYBRGreenI荧光定量PCR/猪肺泡巨噬细胞Key words
Interferon inducible transmembrane protein 3/Porcine reproductive and respiratory syndrome virus/real time SYBR Green I RT-PCR/porcine alveolar macrophages分类
农业科技引用本文复制引用
李玉明,马志永,武专昌,刘珂,马改妮,魏建超,邵东华,李蓓蓓,邱亚峰,石元元..PRRSV感染对猪肺泡巨噬细胞IFITM3基因转录的影响[J].中国动物传染病学报,2018,26(1):7-12,6.基金项目
973项目计划课题(2014CB542702) （2014CB542702）
国家自然科学基金(31502046) （31502046）
