中国动物传染病学报2018，Vol.26Issue(1)：13-18,6.
银杏外种皮多糖抑制J亚群禽白血病病毒在DF-1细胞中的复制
ANTIVIRAL ACTIVITY OF GINKGO BILOBA EXOCARP POLYSACCHARIDE AGAINST SUBGROUP J AVIAN LEUKOSIS VIRUS IN DF-1 CELL
摘要
Abstract
To investigate the antiviral effects of ginkgo biloba exocarp polysaccharide (GBEP) on the replication of Avian leukosis virus subgroup J (ALV-J), DF-1 cells were treated with GBEP at different time points. The results indicated that GBEP strongly inhibited viral gene transcription, protein expression and virus titers through dose and time dependent manners as detected using qRT-PCR, Western blot and TCID50assays. We also noted that GBEP greatly down-regulated the expression of IL-1β, TNFα and other immune related cytokines. Overall, this study highlighted that GBEP exerted its anti-avian leukosis virus activity and might be developed as a preventive drug for ALV-J infection in the future.关键词
J亚群禽白血病病毒/银杏外种皮多糖/病毒复制/抑制Key words
Avian leukosis virus subgroup J/ginkgo biloba exocarp polysaccharide/virus replication/inhibition分类
农业科技引用本文复制引用
钱琨,孔正茹,张杰,秦爱建..银杏外种皮多糖抑制J亚群禽白血病病毒在DF-1细胞中的复制[J].中国动物传染病学报,2018,26(1):13-18,6.基金项目
国家重点研发计划(2017YFD0500700、2016YFD0500800) （2017YFD0500700、2016YFD0500800）
国家自然科学基金(31772734) （31772734）
江苏省优势学科项目 （）
扬州大学新世纪人才项目 （）
