中国动物传染病学报2018，Vol.26Issue(1)：43-47,5.
抗Iss抗血清抑制鸡大肠杆菌血清抗性的分析
MECHANISM OF ANTI-ISS SERUM AGAINST AVIAN PATHOGENIC ESCHERICHIA COLI
摘要
Abstract
The iss gene is the virulence gene of avian E.coli,which is related to its serum resistance.However,there are few reports regarding immune protection of the Iss protein. In the present study, the GST-Iss fusion protein was used to immunize 4-6 week-old mice. The indirect ELISA was used to detect the titer of the antiserum and the expression of Iss protein in pathogenic E.coli strains CVCC1553 and O2.The changes of serum resistance and mRNA transcription level were detected in these two strains as detected using in vitro serum resistance inhibition and real-time fluorescence quantitative PCR assay for iss,ybtA,chuA genes.The results concluded that the anti-Iss serum inhibited the serum resistance of O2 and CVCC1553 strains in vitro and reduced the transcription of iss,ybtA and chuA genes in different serotype strains. The mechanism for the anti-Iss serum may be due to its role in decreasing the serum resistance of pathogenic bacteria by reducing the transcription of the serum resistance related genes. In addition, Iss protein may have the potential for development of the subunit vaccine.关键词
抗Iss抗血清/血清抗性/转录Key words
Anti-Iss serum/serum resistance/transcription分类
农业科技引用本文复制引用
樊琛,曾庆华,王会,李燕,刘桂芹,孙小凡,郑焕芹..抗Iss抗血清抑制鸡大肠杆菌血清抗性的分析[J].中国动物传染病学报,2018,26(1):43-47,5.基金项目
国家自然科学基金青年项目(31302128) （31302128）
聊城大学博士科研启动基金(31805) （31805）
