中国心理卫生杂志2017，Vol.31Issue(11)：916-920,5.DOI:10.3969/j.issn.1000-6729.2017.11.016
Znf804a基因在发育期鼠脑神经元的迁移、增殖及分化中的功能
Function of Znf804a in neuronal migration, proliferation and differentiation during mice brain development
陈天达 1吴秦伟 1卢天兰 1岳伟华 1张岱1
作者信息
- 1. 北京大学第六医院,北京大学精神卫生研究所,国家精神心理疾病临床医学研究中心(北京大学第六医院),卫生部精神卫生学重点实验室(北京大学),北京100191
- 折叠
摘要
Abstract
Objective:To explore the function of Znf804a during brain development by using mouse model.Methods:The shRNA of Znf804a (shZnf804a) and control (pSUPER) plasmids were introduced into ventricular zone of ICR (Institute of Cancer Research) mice at E14.5 (three mice in each group) by using in utero electroporation.The speed of migration was evaluated by comparing the proportions of neuron in cortical plate (CP) zone.The proliferation speed was evaluated by comparing the diameters of neurospheres formed by neuron progenitor cells.The differentiation speed was evaluated by comparing the proportions of Nestin staining positive cells in neuron progenitor cells.Results:The proportion of neurons in CP zone was lower in shZnf804a group than in controls(11.8％ vs.75.4％,P ＜ 0.001).The diameter of neurospheres formed by neuron progenitor cells was bigger in shZnf804a group than in controls (295μm vs.172μm,P ＜0.01).The proportion of Nest in staining positive cells in neuron progenitor cells was larger in shZnf804a group than in controls (31.5％ vs.9.6％,P ＜0.01).Conclusion:It suggests that the migration speed of neurons in shZnf804a is lower than that in controls,the proliferation speed is higher than that in controls,and the differentiation speed is lower than that in controls.These results indicate that Znf804a may play an important role in the development of mouse brain.关键词
脑发育/神经元迁移/增殖/Znf804aKey words
brain development/neuronal migration/proliferation/Znf804a分类
生物科学引用本文复制引用
陈天达,吴秦伟,卢天兰,岳伟华,张岱..Znf804a基因在发育期鼠脑神经元的迁移、增殖及分化中的功能[J].中国心理卫生杂志,2017,31(11):916-920,5.
