中国药房2017，Vol.28Issue(30)：4260-4264,5.DOI:10.6039/j.issn.1001-0408.2017.30.22
近红外漫反射光谱法结合PLS法快速测定红景天药材中水分和红景天苷的含量
Rapid Determination of Moisture and Salidroside in Rhodiolae crenulatae by NIR Combined with PLS Algorithm
摘要
Abstract
OBJECTIVE:To develop a method for rapid determination of moisture and salidroside in Rhodiolae crenulatae.METHODS:The content of moisture was determined by the methods of oven drying (as reference value);the content of salidroside was determined by HPLC (as reference value).Near infrared spectroscopy (NIR) combined with partial least squares (PLS) algorithm were adopted to establish quantitative model for the contents of moisture and salidroside.According to reference value,83 samples were collected for preprocessing spectra by Savitsky Golay with second derivative and Norris Derivative with first derivative.The optimal spectrum range of moisture and salidroside were 7 343.60-6 865.34,5 183.72-4 890.59 cm-1 and 7 050.47-4 327.48 cm-1,respectively.RESULTS:The content determination methodology of salidroside was in line with the requirement.The correlation coefficients of calibration for moisture and salidroside were 0.968 9 and 0.920 3,and the root mean square errors of calibration were 0.273 0 and 0.085 0,respectively.The correlation coefficients of predication were 0.977 1 and 0.930 0,and the root mean square errors of prediction were 0.222 0 and 0.075 5,respectively.CONCLUSIONS:The method is proved to be simple,rapid,non-destructive and accurate,which can be used for the rapid determination of moisture and salidroside in R.crenulatae.关键词
近红外漫反射光谱技术/红景天/水分/红景天苷/偏最小二乘法Key words
Near infrared spectroscopy/Rhodiolae crenulatae/Moisture/Salidroside/PLS分类
医药卫生引用本文复制引用
牟倩倩,贺敬霞,张建琪,杨滨,李小芳,刘江弟,唐艳,李化..近红外漫反射光谱法结合PLS法快速测定红景天药材中水分和红景天苷的含量[J].中国药房,2017,28(30):4260-4264,5.基金项目
中医药行业科研专项项目(No.国中医药科技发[2014]10号) （No.国中医药科技发[2014]10号）
