中国药房2017，Vol.28Issue(30)：4265-4268,4.DOI:10.6039/j.issn.1001-0408.2017.30.23
HPLC法同时测定参柏舒心颗粒中4种成分的含量
Simultaneous Determination of 4 Components in Shenbai Shuxin Granules by HPLC
摘要
Abstract
OBJECTIVE:To establish a method for simultaneous determination of sodium danshensu,protocatechuic aldehyde,salvianolic acid B and phellodendrine hydrochloride in Shenbai shuxin granules.METHODS:HPLC method was adopted.The separation was performed on Waters sunfire-C18 column with mobile phase consisted of acetonitrile-0.05 ％ trifluoroacetic acid (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was set at 288 nm,and the column temperature was 30 ℃.The sample size was 5 μL.RESULTS:The linear ranges of sodium danshensu,protocatechuic aldehyde,salvianolic acid B and phellodendrine hydrochloride were 0.040 01-1.600 46 μg(r=0.999 9),0.013 84-0.553 7 μg(r=0.999 9),0.049 32-1.972 94 μg(r=0.999 6),0.014 46-0.578 6 μg(r=0.999 8).The limits of quantitation were 7.68,2.66,4.74,1.38 ng,and the limits of detection were 1.92,0.66,2.36,0.69 ng,respectively.RSDs of precision,stability and reproducibility tests were all lower than 2％.The recoveries were 98.846％-100.762％ (RSD=0.77％,n=6),96.632％-99.463％ (RSD=0.98％,n=6),98.541％-100.432％ (RSD=0.82 ％,n =6),98.607 ％-101.521％ (RSD =1.11％,n =6),respectively.CONCLUSIONS:The method is simple,accurate and precise.It can be used for 4 components in Shenbai shuxin granules.关键词
参柏舒心颗粒/含量测定/丹参素钠/原儿茶醛/丹酚酸B/盐酸黄柏碱/高效液相色谱法Key words
Shenbai shuxin granules/Content determination/Sodium danshensu/Protocatechuic aldehyde/Salvianolic acid B/Phellodendrine hydrochloride/HPLC分类
医药卫生引用本文复制引用
肖春霞,文永盛,周世玉..HPLC法同时测定参柏舒心颗粒中4种成分的含量[J].中国药房,2017,28(30):4265-4268,4.基金项目
国家药品标准提高研究课题(No.财社[2015]77号) （No.财社[2015]77号）
