中国药房2017，Vol.28Issue(30)：4268-4271,4.DOI:10.6039/j.issn.1001-0408.2017.30.24
HPLC法同时测定干髓糊剂中丁香酚和麝香草酚的含量
Simultaneous Determination of Eugenol and Thymol in Mummification Pastes by HPLC
摘要
Abstract
OBJECTIVE:To establish a method for simultaneous determination of eugenol and thymol in mummification pastes.METHODS:HPLC method was adopted.The determination was performed on Wonda Cract C18 column with mobile phase consisted of methanol-0.2％ phosphoric acid (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was 274 nm,and column temperature was set at 25 ℃.The sample size was 10 μL.RESULTS:The linear ranges of eugenol and thymol were 0.02-0.19 mg/mL (r=0.999 7) and 0.03-0.30 mg/mL (r=0.999 6).RSDs of precision,stability and reproducibility tests were all lower than 2.0％.The recoveries were 95.85％-99.84％(RSD=1.11％,n=9) and 95.81％-100.91％(RSD=1.59％,n=9).CONCLUSIONS:The method is simple and accurate,and can be applied to simultaneous determination of eugenol and thymol in mummification pastes.关键词
高效液相色谱法/干髓糊剂/丁香酚/麝香草酚/含量测定Key words
HPLC/Mummification pastes/Eugenol/Thymol/Content determination分类
医药卫生引用本文复制引用
曹伟宇,贺文娟,雷丸,王晓娟..HPLC法同时测定干髓糊剂中丁香酚和麝香草酚的含量[J].中国药房,2017,28(30):4268-4271,4.基金项目
军队医疗机构制剂标准提高研究项目(No.13ZJZ08) （No.13ZJZ08）
