中国药房2017，Vol.28Issue(30)：4271-4274,4.DOI:10.6039/j.issn.1001-0408.2017.30.25
UPLC-MS法检测动物类药材中氯霉素类药物残留
Determination of Chloramphenicol Residue in Animal Medicinal Herbs by UPLC-MS
摘要
Abstract
OBJECTIVE:To establish the method for determination of chloramphenicol residue in animal medicinal herbs.METHODS:UPLC-MS method was adopted.The determination was performed on SB-C18 column with mobile phase consisted of acetonitrile-water (gradient elution) at the flow rate of 0.2 mL/min.The column temperature was 35 ℃,and sample size was 20 μL.The ion source was a jet stream ion focusing electrospray ion source.The temperature and flow of drying gas were 350 ℃ and 5 L/ min,and those of sheath gas were 250 ℃,11 L/min,and capillary voltage was 3 500 V.Negative ion scanning mode was conducted with multiple reaction monitoring (MRM) mode.RESULTS:The linear ranges of thiamphenicol,florfenicol and chloramphenicol were 0.5-15 ng/mL(r were 0.999 8,0.999 9,0.998 9,respectively).The limits of quantitation were 0.03,0.03,0.15 μg/kg,and the limits of detection were 0.01,0.01,0.05 μg/kg.RSDs of precision,stability and reproducibility tests were all lower than 3.0％.The recoveries were 84.00％-112.80％ (RSD=10.15％,n=9),88.24％-109.80％ (RSD=7.11％,n=9),88.24％-99.02％ (RSD=3.91％,n=9). CONCLUSIONS:The method is simple,precise,stable and reproducible,and can be used for determination of chloramphenicol in animal medicinal herbs.关键词
动物类药材/氯霉素类药物残留/超高效液相色谱-质谱法Key words
Animal medicinal herbs/Chloramphenicol residue/UPLC-MS分类
医药卫生引用本文复制引用
张治军,周文杰,李志芸..UPLC-MS法检测动物类药材中氯霉素类药物残留[J].中国药房,2017,28(30):4271-4274,4.基金项目
广西壮族自治区食品药品监督管理局科研项目(No.桂食药监科评函[2015]20号) （No.桂食药监科评函[2015]20号）
