中国药房2017，Vol.28Issue(30)：4275-4277,3.DOI:10.6039/j.issn.1001-0408.2017.30.26
HPLC-ELSD法同时测定复方小酸枣咀嚼片中4种指标性成分的含量
Simultaneous Determination of 4 Active Components in Compound Xiaosuanzao Chewable Tablets by HPLC-ELSD
摘要
Abstract
OBJECTIVE:To establish the method for the content determination of 4 active components in Compound xiaosuanzao chewable tablets.METHODS:HPLC-ELSD method was adopted.The determination was performed on a Grace Brava C18-BDS column with mobile phase consisted of acetonitrile-water (gradient elution) at the flow rate of 1.0 mL/min.The column temperature was 25 ℃,and sample size was 20 μL.The drift tube temperature is 100 ℃,and the carrier gas flow rate is 2.9 L/min.RESULTS:The linear ranges of betulic acid,betulinol,pachymic acid and glycyrrhizic acid were 44.50-890.0 μg/mL (r=0.999 3),20.28-405.6 μg/mL (r=0.999 7),20.50-656.0 μg/mL(r=0.999 7) and 10.50-336.0 μg/mL(r=0.999 6),respectively.RSDs of precision,stability and reproducibility were all lower than 3.0％.The recoveries were 99.44％-101.12％ (RSD=0.57％,n=6),99.41％-100.39％ (RSD=0.34％,n=6),99.31％-100.46％ (RSD=0.51％,n=6),98.96％-101.19％ (RSD=0.84％,n=6),respectively.CONCLUSIONS:The method is simple,precise,stable and reproducible,and can be used for simultaneous determination of 4 active components in Compound xiaosuanzao chewable tablets.关键词
高效液相色谱-蒸发光散射法/复方小酸枣咀嚼片/白桦脂酸/白桦脂醇/茯苓酸/甘草酸Key words
HPLC-ELSD/Compound xiaosuanzao chewable tablets/Betulic acid/Betulinol/Pachymic acid/Glycyrrhizic acid分类
医药卫生引用本文复制引用
张立峰,刘灿仿,张东平,陈亚,卫茹,褚燕..HPLC-ELSD法同时测定复方小酸枣咀嚼片中4种指标性成分的含量[J].中国药房,2017,28(30):4275-4277,3.基金项目
河北省高等学校科学研究项目(No.ZC2016121) （No.ZC2016121）
