中国药房2017，Vol.28Issue(30)：4285-4288,4.DOI:10.6039/j.issn.1001-0408.2017.30.29
苗药朱砂根的HPLC指纹图谱研究
Study on HPLC Fingerprint of Miao Medicine Ardisia crenata
摘要
Abstract
OBJECTIVE:To establish HPLC fingerprints of Miao medicine Ardisia crenata.METHODS:HPLC method was adopted.The determination was performed on Diamonsil C18 column with mobile phase consiste of methanol-water (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was 220 nm,and column temperature was maintained at 30 ℃.The sample size was 10 μL.Using 11-O-(3',4',5'-three-o-galloylhyperin)-bergeninum as reference,HPLC fingerprints of 16 batches of samples were determined.Common identification and similarity evaluation were performed by using TCM Chromatographic Fingerprint Similarity Evaluation Software (2012 edition).Cluster analysis of fmgerprrints was conducted.RESULTS:There were 6 common peaks in HPLC fingerprints of 16 batches of samples.The similarity among 8 batches was more than 0.9.The 16 batches of samples could be clustered into 4 categories.CONCLUSIONS:Established fingerprints can provide reference for identification and quality evaluation ofA.crenata.关键词
朱砂根/高效液相色谱法/指纹图谱/聚类分析Key words
Ardisia crenata/HPLC/Fingerprint/Cluster analysis分类
医药卫生引用本文复制引用
孙绪,姚成芬,付思红,巩仔鹏,刘亭,杨畅,查俊,李勇军..苗药朱砂根的HPLC指纹图谱研究[J].中国药房,2017,28(30):4285-4288,4.基金项目
贵州省科技计划项目(No.黔科合平台人才[2016]5613、5677) （No.黔科合平台人才[2016]5613、5677）
贵州省教育厅项目(No.黔教合协同创新字[2013]04、黔科合KY字[2013]122、黔教研合ZYRC字[2014]012) （No.黔教合协同创新字[2013]04、黔科合KY字[2013]122、黔教研合ZYRC字[2014]012）
贵州省优秀青年科技人才培养对象专项(No.黔科合人字[2015]11号) （No.黔科合人字[2015]11号）
