中国医科大学学报2017，Vol.46Issue(10)：869-873,5.DOI:10.12007/j.issn.0258-4646.2017.10.002
超声作用下2种压电陶瓷多孔支架材料上成骨细胞的增殖与分化
Effect of Low-intensity Pulsed Ultrasound on the Proliferation and Differentiation of Osteoblasts Cultured on Two Piezoelectric Ceramic Porous Scaffolds
摘要
Abstract
Objective To evaluate the effects of low-intensity pulsed ultrasound (LIPUS) treatment on the proliferation and differentiation of osteoblasts cultured on barium titanate/titanium alloy (BaTiO3) and zinc oxide/titanium alloy (ZnO) piezoelectric ceramic composite scaffolds.Methods The BaTiO3 and ZnO porous composite scaffolds were randomly divided into ultrasound (UBaTiO3 and UZnO) and control (CBaTiO3 and CZnO)groups,and MC3T3-E1 cells were cultured on the surface of the materials in each group.Cells in the ultrasound groups were subjected to a 20-min treatment LIPUS session daily,and the control group was sham-treated without the power source.The proliferation and differentiation rates of osteoblasts were evaluated using methyl thiazolyl tetrazolium (MTT),alkaline phosphatase (ALP),and osteocalcin (OCN) assays.Results Significant differences in the MTF values were observed between the UBaTiO3 and CBaTiO3 groups at day 4 (P ＜ 0.05) and between the UBaTiO3 and UZnO groups at days 1,4,and 7 (P ＜ 0.05).Compared with the CBaTiO3 group,the UBaTiO3 group showed significantly higher ALP activity and OCN levels at days 4 and 7 (P ＜ 0.05).Further,a significant difference in the ALP and OCN values between the UBaTiO3 and UZnO groups (P ＜ 0.05) was observed.Conclusion LIPUS promotes the proliferation and differentiation of osteoblasts on BaTiO3 and ZnO scaffolds.BaTiO3 scaffolds enable better promotion of osteogenesis under LIPUS treatment than ZnO scaffolds do.关键词
低强度脉冲超声波/成骨细胞/压电陶瓷/压电效应Key words
low-intensity pulsed ultrasound/osteoblast/piezoelectric ceramic/piezoelectric effect分类
医药卫生引用本文复制引用
全泉,矫义来,曹洪娟,吴琳..超声作用下2种压电陶瓷多孔支架材料上成骨细胞的增殖与分化[J].中国医科大学学报,2017,46(10):869-873,5.基金项目
国家高技术研究发展计划(863计划)(2015AA033702) （863计划）
辽宁省自然科学基金(2014021008) （2014021008）
