中国医科大学学报2017，Vol.46Issue(10)：874-877,886,5.DOI:10.12007/j.issn.0258-4646.2017.10.003
miR-429通过靶向作用Sox2抑制骨肉瘤干细胞特性
miR-429 Negatively Regulates Osteosarcoma Stem Cell-like Properties by Targeting Sox2
摘要
Abstract
Objective To investigate the role of miR-429 in osteosarcoma stem cells.Methods We employed different approaches to test the expression of miR-429 in osteosarcoma stem cells.miR-429-high and miR-429-low cells were separated using molecular probes and their stem cell-like properties were compared.The effect of miR-429 on osteosarcoma stem cell-like properties was further tested through transfection assays.Furthermore,the miR-429 downstream target gene was confirmed by luciferase assay.Results The expression of miR-429 in osteosarcoma stem cells was much lower than that in control cells.miR-429-high cells displayed fewer stem celllike properties than did miR-429-low cells.These observations were confirmed by transfection assays.Additionally,our luciferase assays showed that miR-429 regulates Sox2 at the post-transcriptional level.Conclusion miR-429 negatively regulates osteosarcoma stem celllike properties by targeting Sox2.关键词
骨肉瘤/干细胞/miRNA-429/Sox2Key words
osteosarcoma/stem cell/miR-429/Sox2分类
医药卫生引用本文复制引用
余铃,张政佩,孙祥然,郑迪,郭卫春..miR-429通过靶向作用Sox2抑制骨肉瘤干细胞特性[J].中国医科大学学报,2017,46(10):874-877,886,5.基金项目
国家自然科学基金(8150257) （8150257）
中央高校基本科研基金(2042015kf0069) （2042015kf0069）
