中国医科大学学报2017，Vol.46Issue(11)：1013-1018,6.DOI:10.12007/j.issn.0258-4646.2017.11.012
细胞DNA定量分析在宫颈病变筛查中的应用价值
Application Value of Quantitative Analysis of Cell DNA in Cervical Lesion Screening
张喆 1孔令婷 1孙寒雪 1李晓晗 1吕庆杰1
作者信息
- 1. 中国医科大学附属盛京医院病理科,沈阳110004
- 折叠
摘要
Abstract
Objective To determine the application value of quantitative detection of DNA in cervical lesion screening.Methods Retrospective analysis of 82 518 cases of cervical lesions was carried out.The sensitivity,specificity,positive predictive value (PPV),and negative predictive value (NPV) of DNA quantification and ThinPrep cytology test (TCT) for the diagnosis of cervical lesions were compared with the results of biopsy.The accuracy of the two methods was evaluated by applying the receiver operating characteristic curve.Results The sensitivity of DNA quantification,TCT,and combined diagnosis was 77.37％,70.97％,and 90.24％,respectively,and the specificity was 65.59％,70.42％,and 45.06％,respectively.The sensitivity of cell DNA quantitative analysis was higher but the specificity was lower than that of TCT,while the sensitivity of combined diagnosis was the highest.In DNA quantitative analysis of patients with and without atypical squamous cells of undetermined significance (ASCUS),the positive rates of pathology were 42.71％ and 12.5％,respectively.The misdiagnosis rate of cell DNA quantitative analysis for screening cervical adenocarcinoma (39.13％) was higher than that for screening cervical squamous cell carcinoma (15.11％).In a cytologic smear,the misdiagnosis rate of cells ＜ 5 000 (57.83％) was much higher than that of cells ＞ 5 000 (22.63％).However,a combined diagnosis with TCT could reduce the misdiagnosis rate from 20.19％ to 13.38％.Conclusion Cell DNA quantification is more sensitive than TCT,but the specificity of cell DNA analysis is lower than that of TCT,and the screening rate for cervical adenocarcinoma is high.Quantitative DNA analysis combined with TCT can significantly improve the screening sensitivity.关键词
细胞DNA定量分析/液基细胞学/宫颈病变Key words
DNA quantitative analysis/ThinPrep cytology test/cervical lesions分类
医药卫生引用本文复制引用
张喆,孔令婷,孙寒雪,李晓晗,吕庆杰..细胞DNA定量分析在宫颈病变筛查中的应用价值[J].中国医科大学学报,2017,46(11):1013-1018,6.
