中国真菌学杂志2017，Vol.12Issue(6)：328-331,336,5.
探讨肺曲霉病患者甘露聚糖结合凝集素(MBL)及T细胞亚群的变化
Detecting mannan-binding lectin and T-lymphocyte subsets for the diagnosis of pulmonary aspergillosis
摘要
Abstract
Objective To investigate the role of mannan-binding lectin (MBL) level and changes of T-lymphocyte subsetson the patients with pulmonary aspergillosis.Methods From May 2013 to May 2015,51 patients were enrolled into disease group,52 healthy adults were enrolled into normalcontrol group.The levels of MBL and Galactomannanwere detected by Enzyme-linked Immunosorbent Assay (ELISA).The percentages of CD3+ CD4+ T cells,CD3+ CD8+T cells,and the ratio of CD3+CD4+/CD3+ CD8+T cells were determined by flow cytometer.Results The express of MBL in disease group (197.96± 148.16 μg/ml) was significantly higher than that of normal group (120.25±98.65)(P＜0.05).The serum galactomannan (GM) level of disease group (0.94±0.77 μg/L) was significantly higher than that of normal group (0.32±0.16 μg/L).The percentages of CD3+CD4+T cells andCD3+CD8+T cells were (33.07±7.9)％,(40.32±7.30)％ (P＜0.05),(33.00±8.29) ％,(25.98±6.65)％(P＜0.05)in disease group and normal group,respectively.The ratios of CD3+ CD4+/CD3+ CD8+T cells in disease group and normal group were 1.09±0.47,1.68±0.65 (P＜0.05),respectively.Conclusion There was significant change of MBL level and the percentages of T lymphocyte subset in patients with pulmonary aspergillosis compared with control group.Immunotherapy may improve the efficacy of pulmonary aspergillosis.Through the change of T-lymphocyte sub set can preliminary assessment the body's immune status in patients,at the same time,providing a theoretical basis for im mune enhancement therapy in pulmonary aspergillosis.关键词
肺曲霉病/甘露糖结合凝集素/T淋巴细胞/免疫Key words
pulmonary aspergillosis/mannan-binding lectin/tlymphocte/immune分类
医药卫生引用本文复制引用
杨燕,滕隔玲,吴艳华,张琳琳,盖丽贞,陈影,胡华..探讨肺曲霉病患者甘露聚糖结合凝集素(MBL)及T细胞亚群的变化[J].中国真菌学杂志,2017,12(6):328-331,336,5.基金项目
山东省科技发展计划项目(2012G0021835) （2012G0021835）
