中国真菌学杂志2017，Vol.12Issue(6)：342-345,4.
HIV感染和非HIV感染人群抗耶氏肺孢子菌主要表面糖蛋白的IgG和IgM抗体水平调查
Quantitative levels of anti-Pneumocystis jirovecii major surface glycoprotein IgG and IgM antibodies in HIV and non-HIV groups
摘要
Abstract
Objective To investigate the quantitative levels of anti-Pneumocystis jirovecii (Pj) major surface glycoprotein (Msg) IgG and IgM antibodies in HIV and non-HIV groups to explore its value in epidemiological investigation.Method One target sequence of Pj Msg gene was selected and expressed using prokaryotic expression system.Enzyme-linked immunosorbent assay (ELISA) was established to detect anti-Pj Msg IgG and IgM antibodies using recombinant Pj Msg as antigen.Multiple proportion dilution methods were established to measure quantitative levels of anti-Pj Msg IgG and IgM antibodies.A cohort of individuals with HIV infection (n =176) and non HIV controls (n =266) in Beijing China were investigated.All patients were not diagnosed with a situation of pneumocystis pneumonia (PCP).Results The positive rate of anti-Pj Msg IgG antibody in non-HIV group was 42.5％ (113/266).Among these 113 positive cases,there were 77 cases (68.1％),15 cases (13.3％),6 cases (5.3％),and 15 cases (13.3％) in the titer of 1 ∶ 100,1 ∶ 200,1 ∶ 400,and 1 ∶ 800 levels respectively.The positive rate of anti-Pj Msg IgG antibody in HIV infection group was 24.4％ (43/176).Among these 43 positive cases,there were 28 cases (65.1％),10 cases (23.3％),3 cases (7.0％),and 2 cases (4.7％) in the titer of 1 ∶ 100,1 ∶ 200,1 ∶ 400,and 1 ∶ 800 levels respectively.There was no statistical significance in the difference of anti-Pj Msg IgG antibody titer levels compared with non-HIV controls (x2 =4.254,P =0.235).The positive rate of anti Pj Msg IgM antibody in non HIV group was 41.7％ (111/266).Among these 111 positive cases,there were 57 cases (51.4％),21 cases (18.9％),19 cases (7.1％),and 14 cases (12.6％) in the titer of 1 ∶ 100,1 ∶ 200,1 ∶ 400,and 1 ∶ 800 levels respectively.The positive rate of anti-Pj Msg IgM antibody in HIV infection group was 12.5％ (22/176).Among these 22 positive cases,there were 10 cases (45.4％),2 cases (9.1％),4 cases (18.2％),and 6 cases (27.3％) in the titer of 1 ∶ 100,1 ∶200,1 ∶ 400,and 1 ∶ 800 levels respectively.There was no statistical significance in the difference of antiPj Msg IgM antibody titer levels compared with non-HIV controls (x2 =3.788,P =0.285).Conclusions Among these non-PCP individuals,the anti-Pj Msg IgG antibody titer levels were mostly between 1 ∶ 100 and 1 ∶ 200.The methods of antibody quantitative levels and their clinic significance need further studies.关键词
耶氏肺孢子菌/主要表面糖蛋白/抗体/定量Key words
Pneumocystis jirovecii/major surface glycoprotein/antibody/quantitative level分类
医药卫生引用本文复制引用
郭爽,刘意,田小军,栗绍刚,宋营改,任翊..HIV感染和非HIV感染人群抗耶氏肺孢子菌主要表面糖蛋白的IgG和IgM抗体水平调查[J].中国真菌学杂志,2017,12(6):342-345,4.基金项目
首都医科大学基础临床科研合作基金(17JL03) （17JL03）
