中国真菌学杂志2017，Vol.12Issue(6)：359-361,367,4.
一种简便、快速、高效适合PCR的真菌DNA提取方法
A rapid and efficient extraction method of fungus DNA for PCR
柴海云 1张磊 1袁牧歌 2朱庆义1
作者信息
- 1. 广州金域医学检验中心微生物室,广州510005
- 2. 广州医科大学金域检验学院,广州511436
- 折叠
摘要
Abstract
Objective To explore a new convenient and efficient fungi DNA extraction method,which could be used in many kinds of fungi.Methods We associated enzymic digestion with Chelex-100 to make up a new reagent for fungi DNA extraction.Results During the experiment,34 strains were tested.The DNAs of 32 strains could be obtained by our reagent,while only 27 strains' DNAs could be extracted by Takara lysis buffer.For the strains we did not successfuly obtain the DNA by the above methods,we grinded the fungi by liquid nitrogen.After that,our reagent obtained their DNA by,and only one by Takara lysis buffer.Conclusion The DNA extraction efficiency of our reagent is much better than that of Takara lysis buffer.It can be used in many kinds of fungi.For some fungi,we should firstly grind it before DNA extraction.关键词
蛋白酶K/蜗牛酶/Chelex-100/DNA提取Key words
Protease-K/snailase/Chelex-100/DNA extraction分类
医药卫生引用本文复制引用
柴海云,张磊,袁牧歌,朱庆义..一种简便、快速、高效适合PCR的真菌DNA提取方法[J].中国真菌学杂志,2017,12(6):359-361,367,4.
