针灸临床杂志2017，Vol.33Issue(10)：56-60,5.
电针对青春期慢性束缚应激模型大鼠不同时段血清TNF-α、IL-1β表达的影响
Impact of Electro-acupuncture on Pubescent CRS Rats:Expressions of TNF-α and IL-1β at Different Time-points
摘要
Abstract
Objective:To investigate the antidepressant effect of electro-acupuncture(EA) on the expressions of TNF-α and IL-1β in the pubescent rats with chronic restraint stress(CRS),and to explore the relative immune mechanism.Methods:60 SD rats (male,five weeks) were randomized into the blank control group (group A,n =20),the model group (group B,n =20),and the electro-acupuncture group (group C,n =20).The rats in group A were bred in groups;CRS models were established by separate breeding combined with chronic restraint stress(immobilization for 6h daily from 9:00 to 15:00);in group C,electro-acupuncture intervention was applied on point GV20 and point GV29 for 20mins one hour before the restraint,once daily for 28 days.From the 28th day to 35th day,there was no treatment application in the three groups.Open-field test was used to assess the behavior of the CRS rats.Serum levels of TNF-α and IL-1β were detected with Bio-Plex method on 7th,28th and 35th day respectively.Results:In terms of scores of the open-filed test,there was no significant difference in the three groups before the experiment;on 28th day,the scores of group C were remarkably increased compared to those of group B (P ＜ 0.01).In terms of serum levels of TNF-α and IL-1β on 7th day,TNF-α level increased considerably in group B compared to that in group A (P ＜ 0.01),but there was no significant differences in IL-1β level between the two groups(P ＞ 0.05);compared to group B,TNF-α expression was down-regulated significantly in group C (P ＜ 0.01).On 28th day,levels of TNF-α and IL-1β increased significantly in group B compared to those in group A (P ＜ 0.01);however,they decreased obviously in group C when compared to group B(P ＜0.01).On 35th day,there were no significant differences in serum levels of TNF-α and IL-1β among the three groups (P ＞ 0.05).Conclusion:CRS can raise TNF-α level and reduce IL-1β level in pubescent rats,and electro-acupuncture can regulate these changes,which may contribute to its antidepressant effect.关键词
电针/青春期/慢性束缚应激/抑郁症/TNF-α/IL-1βKey words
Electro-acupuncture/Puberty/Chronic restraint stress/Depression/TNF-α/IL-1β分类
医药卫生引用本文复制引用
俞秋云,李想,冯世醒,施鹏,图娅,孙岚,姜会梨,王瑜,赵冰骢,杨昕婧,李晶,梁星辰,孙阳..电针对青春期慢性束缚应激模型大鼠不同时段血清TNF-α、IL-1β表达的影响[J].针灸临床杂志,2017,33(10):56-60,5.基金项目
国家自然科学基金,编号:81173334,81373729 （）
北京中医药大学在读研究生项目,编号:2017-JYB-xs-047. （）
