针灸临床杂志2017，Vol.33Issue(10)：60-63,封3,5.
夹脊电针联合甲基强的松龙对ASCI大鼠脊髓组织NMDA受体亚单位NR1蛋白表达的影响
Protein Expression of NMDA-NR1 in Spinal Cord Tissue of ASCI Rats Intervened by Jiaji Electro-acupuncture Combined with Methylprednisolone
摘要
Abstract
Objective:To observe the effect of Jiaji electro-acupuncture combined with Methylprednisolone on the scores of limb motor function and the expression of N-methyl-D-aspartate receptor subunit NR1 (NMDAR-NR1) of spinal cord tissue in ASCI(acute spinal cord injury) rats,and to explore the mechanism of this therapy for ASCT.Methods:72 Wistar rats(male) were randomly divided into the sham group(group A,n =24),the model group(group B,n =24),and Jiaji electro-acupuncture combined with Methylprednisolone group(group C,n =24);each group was subdivided into four subgroups according to 1 d,3 d,7 d and 14 d,with 6 rats in each group.ASCI models were established with NYU striker.The rats in group A were applied with sham operation without modeling followed by conventional breeding;the rats in group B were modeled followed by conventional breeding;whereas,the rats in group C were given one-time injection of Methylprednisolone(30 mg/kg) 30min after modeling,Jiaji electro-acupuncture was applied 3h after the surgery (30 min/time,once per day).The limb motor function was observed by evaluating the scores of BBB,and the expression of NMDA-NR1 was detected by immunohistochemistry.Results:In term of scores of BBB,it was significantly reduced in group B than that in group A(P ＜0.05);however,it was remarkably increased after three -day treatment in group C compared to that in group B(P ＜0.05).In terms of NMDA-NR1 expression,it was significantly increased in group B compared to that in group A (P ＜ 0.05);but it was obviously decreased after three-day treatment in group C compared to that in group B(P ＜ 0.05).Conclusion:NMDA-NR1 may significantly increased in ASCI rats,and the therapy of Jiaji electro-acupuncture combined with Methylprednisolone can down-regulate NMDA-NR1,which may be one of its mechanisms to improve limb motor function in ASCI rats.关键词
急性脊髓损伤/夹脊电针/甲基强的松龙/N-甲基-D-天冬氨酸受体Key words
Acute spinal cord injury/Jiaji electro-acupuncture/Methylprednisolone/NMDAR分类
医药卫生引用本文复制引用
李晓宁,吴磊,覃业校,梁雪松,单筱淳,梅继林,李诺..夹脊电针联合甲基强的松龙对ASCI大鼠脊髓组织NMDA受体亚单位NR1蛋白表达的影响[J].针灸临床杂志,2017,33(10):60-63,封3,5.基金项目
国家自然科学基金项目,编号:81373715 （）
哈尔滨市应用技术研究与开发项目科技创新人才类(优秀学科带头人B类)项目,编号:2016RAXYJ090. （优秀学科带头人B类）
