中国现代中药2017，Vol.19Issue(9)：1221-1227,7.DOI:10.13313/j.issn.1673-4890.2017.9.003
柬埔寨民间习用灵芝与伴生真菌拮抗特性评价
Evaluation of Antagonistic Effect between Ganoderma Isolates and Companion Fungi from Cambodia
摘要
Abstract
Objective:To isolate and identify ferine G anoderma isolates in Cambodia and its companion fungi,and evaluate the influence of companion fungi by antagonistic test.Methods:64 fungal strains from the folk used Ganoderma in Cambodia were investigated by analysis of ITS sequences.The relationship of Ganoderma and companion fungus which came from the same fruiting body were judged by antagonistic effect.Results:22 isolates were Ganoderma,in the proportion of 34.4％,including 19 Ganoderma lingzhi and 3 Ganoderma sichuanense.Xylaria and Hypoxylon were most commonly in nonGanoderma,in the proportion of 35.9％ and 15.6％,respectively.In addition,a Ganoderma pathogen was isolated and identified as Xylogone ganodermophthora,a medicinal fungus was isolated and identified as lrpex lacteus.It is proved that the antagonistic effect was more evident among Ganoderma isolates,but interwoven growth occurred between Ganoderma and companion fungus,this will affect the quality of Ganoderma.Conclusion:The medicinal properties still need professional and systematic research of the folk used ferine Ganoderma in Cambodia,and then be able to established as a new natural distribution or producing area of medicinal Ganoderma germplasm or resources.The native Xylaria and storage mode are more important factors to affecting the quality or Geo-herbalism of Cambodia Ganoderma.关键词
柬埔寨/野生灵芝菌株/ITS序列/伴生真菌/拮抗/品质Key words
Cambodia/ferine Ganoderma isolates/ITS/companion fungus/antagonistic test/quality引用本文复制引用
佟曦然,郭豪杰,罗丽,张昭,齐耀东,张本刚..柬埔寨民间习用灵芝与伴生真菌拮抗特性评价[J].中国现代中药,2017,19(9):1221-1227,7.基金项目
中国医学科学院医学与健康科技创新工程项目(2016-I2M-2-003) （2016-I2M-2-003）
