中国现代中药2017，Vol.19Issue(9)：1245-1248,4.DOI:10.13313/j.issn.1673-4890.2017.9.007
靶细胞萃取联用UPLC/Q-TOF-MS方法分析川芎中的药效成分
Analysis of Pharmacodynamic Components in Chuanxiong Rhizoma by Target Cell Sorption Combined with UPLC/Q-TOF-MS
摘要
Abstract
Objective:To establish a method by using target cell and UPLC/Q-TOF-MS for extraction the pharmacodynamic components in Chuanxiong Rhizoma.Methods:The human umbilical vein endothelial cells (HUVEC) were used as target cells,and the freeze-dried powder of Chuanxiong Rhizoma in logarithmic growth period was added.After 2 h incubation,the cells were collected and frozen rapidly with liquid nitrogen,the cells were broken by repeated freezing and thawing,and centrifuged to obtain a sample of cells after extraction.While compounds in the extract of cell samples were determined by UPLC/QTOF-MS,the effect of possible effective components that entered the cell of Chuanxiong Rhizoma was analyzed.Results:After HUVEC extraction two active ingredients ferulic acid and ligustilide were obtained.At the same time,the method was established for the determination of the two components.Conclusion:The analysis method of the target cell extraction and UPLC/QTOF-MS can be applied for screening out the active ingredients in Chuanxiong Rhizoma by specific binding effect between the compound and the target (receptor,channel,enzyme,etc.in the cell,so as to provide a new method for screening the active ingredients and drug mechanism study.关键词
川芎/靶细胞萃取/藁本内酯/阿魏酸/UPLC/Q-TOF-MSKey words
Chuanxiong Rhizoma/target cell sorption/ferulic acid/ligustilide/UPLC/Q-TOF-MS引用本文复制引用
谢佳宾,王媛,田梦,李亚梅,武媛媛,王玉明,李遇伯,张艳军..靶细胞萃取联用UPLC/Q-TOF-MS方法分析川芎中的药效成分[J].中国现代中药,2017,19(9):1245-1248,4.基金项目
国家科技支撑计划课题(2011BAI07B08) （2011BAI07B08）
