中国现代中药2017，Vol.19Issue(9)：1249-1253,5.DOI:10.13313/j.issn.1673-4890.2017.9.008
华蟾酥毒基对U2OS细胞增殖和凋亡的影响
Effect of Cinobufagin on Proliferation and Apoptosis of U2OS
摘要
Abstract
Objective:To investigate the effect of cinobufagin on the growth inhibition,apoptosis and the expression of Caspases mRNA in human osteosarcoma cell line U2OS.Methods:Osteosarcoma cell line U2OS was cultured in vitro.Cell proliferation was detected by Cell Counting Kit-8 (CCK-8).The cells stained by Annexin V and PI were observed by flow cytometry (FCM) for apoptosis.Hoechst 33258 was used to detect the morphological change of apoptotic cells.The mRNA expression of Caspases in U2OS was observed by RT-PCR.Results:The proliferation of human osteosarcoma cell line U2OS could be inhibited and apoptosis could be induced by cinobufagin.The inhibition rate and apoptosis rate were different with the different concentrations.The inhibition rate and apoptosis rate of U2OS cells rose significantly when the concentration of cinobufagin was over 20 nmol · L-1.Apoptotic body could be observed by Hoechst 33258 dying.The Caspases mRNA expression of human osteosarcoma cell line U2OS increased obviously by using cinobufagin.Conclusion:Cinobufagin could significantly inhibit the proliferation and induce apoptosis of human osteosarcoma cell line U2OS.Its mechanism of action may be related to activation of Caspase dependent apoptosis pathway.关键词
华蟾酥毒基/骨肉瘤/细胞增殖/细胞凋亡Key words
Cinobufagin/osteosarcoma/proliferation/apoptosis引用本文复制引用
余铃,郭卫春,代国..华蟾酥毒基对U2OS细胞增殖和凋亡的影响[J].中国现代中药,2017,19(9):1249-1253,5.基金项目
国家自然科学基金(81341078) （81341078）
