中国现代中药2017，Vol.19Issue(9)：1254-1261,8.DOI:10.13313/j.issn.1673-4890.2017.9.009
何首乌及其主要成分二苯乙烯苷对非酒精性脂肪肝大鼠肠道短链脂肪酸产生量的影响
Effect of Radix Polygoni Multiflori and TSG on Short-chain Fatty Acids in Intestinal Tract of NAFLD Rats
摘要
Abstract
Objective:To study the effect of Radix Polygoni Multiflori and its active ingredient,TSG,on shortchain fatty acids (SCFA),which were gut microbiota fermentation products of carbohydrates,in rats with nonalcoholic fatty liver disease.Methods:SD rats were randomly distributed into seven groups,mice were fed with either standard chow diet or high-fat diet,plus daily administration of water or water extract of Radix Polygoni Multiflori or TSG.The contents of acetic acid,propionic acid and butyric acid in stool samples were determined by gas chromatography.TC,TG contents in liver and blood sample,and endotoxin contents in hepatic portal venous blood were tested.Results:The water extract of Radix Polygoni Muhiflori and TSG could reduce the contents of total SCFA in the intestinal tract of NAFLD mice fed by HFD,however,gender differences might existed.PMR and TSG could reduce acetic acid,propionic acid,butyric acid in HFD-fed male mice.At the same time,they relived high contents of TG,TC in liver tissue and LPS level in portal venous.In female rats,the propionic acid content was lower in PMR group compared with NAFLD rats.PMR also decreased the TC、TG level in hepatic tissues of female rats.However,low dose TSG raised acetic acid content,while reduced blood lipid and LPS level.In the meantime,regulation effect of PMRP on intestinal SCFA content of both female and male mice was not significant.Conclusion:Radix Polygoni Muhiflori and its active ingredient TSG affected SCFA level produced by gut microbial fermentation.This regulatory activity may contribute to their treatment activity to NAFLD.关键词
何首乌/二苯乙烯苷/短链脂肪酸/非酒精性脂肪肝/气相色谱Key words
Radix Polygoni Multiflori/TSG/short-chain fatty acids/non-alcoholic fatty liver/gas chromatography引用本文复制引用
王艳芳,林佩,陆建美,张美,李莉,杨兴鑫,俞捷..何首乌及其主要成分二苯乙烯苷对非酒精性脂肪肝大鼠肠道短链脂肪酸产生量的影响[J].中国现代中药,2017,19(9):1254-1261,8.基金项目
国家自然科学基金(81060337,81460623) （81060337,81460623）
云南省应用基础研究重点项目(2014FA035) （2014FA035）
云南省教育厅南药协同创新中心项目(30270100500) （30270100500）
云南省中青年学术和技术带头人后备人才项目(2015HB053) （2015HB053）
云南省应用基础研究计划项目青年基金(2016FD050) （2016FD050）
