中国现代中药2017，Vol.19Issue(9)：1262-1269,8.DOI:10.13313/j.issn.1673-4890.2017.9.010
基于微量量热法的不同来源黄芩抑菌活性对比研究
Comparative Study on Bacteriostatic Activity of different Scutellaria baicalensis Based on Microcalorimetric Technique
摘要
Abstract
Objective:To study the bacteriostatic activity of different Scutellaria baicalensis based on the microcalorimetric technique.Methods:The effects of different S.baicalensis on the thermokinetics parameters of Escherichia coli metabolism were studied by using the technology of microcalorimetric,and the differences of bacteriostatic activity of S.baicalensis on different cultivation area,harvesting time,growing year and germplasm were compered.Results:The results showed the differences about thermokinetics parameters among the differences cultivation area.The bacteriostatic activity changed between 24.3％ and 129.5％,and the samples root from Hebei and Neimeng showed stronger bacteriostatic activity than those from Shangdong and Shangxi.It exhibited the first declining later increasing of bacteriostatic activity with the harvesting time changing (from the July to the November),and the strongest bacteriostatic activity occurred in November.There were also the trends of gradually increase on the bacteriostatic activity with the increasing years of planting (from two years to five years).The experimental result of multi-site testes showed a big difference among the different plant germplasm,it should be a relevance between bacteriostatic activity and the spanning of latitude across.Conclusion:There exist some differences among these samples from difference cultivated area and the harvesting time,growing years and germplasm should also affect the bacteriostatic activity.It is concluded that the method based on the microcalorimetric technique could be used to distinguish the different S.baicalensis,and is more convenient and faster,also provide more information for distinguish pharmacological activity of different drug sources.关键词
黄芩/微量量热法/生物活性/品质区分Key words
Scutellaria baicalensis Georgi/microcalorimetric/biological activity/quality distinguish引用本文复制引用
曾燕,郭兰萍,王继永,黄璐琦..基于微量量热法的不同来源黄芩抑菌活性对比研究[J].中国现代中药,2017,19(9):1262-1269,8.基金项目
中医药行业科研专项(201407005-5,201507002) （201407005-5,201507002）
中国中药公司博士后专项(2012) （2012）
江苏省中药资源产业化过程协同创新中心项目(2013) （2013）
