中国现代中药2017，Vol.19Issue(9)：1296-1300,5.DOI:10.13313/j.issn.1673-4890.2017.9.017
超声波协同复合酶提取菟丝子总黄酮工艺优化
Extraction Optimization of Total Flavonoids from Cuscuta chinensis with Compound Enzyme under Ultrasonic Wave
摘要
Abstract
Objective:Total flavonoids from Cuscuta chinensis were extracted with compound enzyme under ultrasonic wave aiming at improving the rate of extraction.Methods:The pH,temperature and hydrolysis time were selected as influencing factors based on single factors,and the extraction rate of total fiavonoids was selected as response value.The effects of all variables and their interactions on extraction rate of total flavonoids from C.chinensis were investigated through Box-Behnken experiment design method.Results:The optimum extraction conditions of total flavonoids were as follows:pH 4.05,temperature at 52.6 ℃,and ultrasonic time of 20.3 min.Under the optimized conditions,the maximum extraction yield of total flavonoids was 23.39 mg · g-1.Conclusion:The optimized extraction process has the advantages of less time,easy operation,the higher rate of the extraction of total flavonoids.关键词
菟丝子总黄酮/复合酶/超声波/响应面分析法Key words
Total flavonoid from Cuscuta chinensis/enzyme hydrolysis/ultrasonic/response surface analysis引用本文复制引用
孙艳玲,李泽琦,冯冠英,郑彧..超声波协同复合酶提取菟丝子总黄酮工艺优化[J].中国现代中药,2017,19(9):1296-1300,5.基金项目
辽宁省大学生创新创业训练计划项目(201510162000048) （201510162000048）
