中医药学报2017，Vol.45Issue(5)：10-13,4.
知母、麦冬和薯蓣中螺甾体皂苷的质谱裂解规律研究
Fragmentation Patterns of Five Spirostanol Steroidal Saponins
摘要
Abstract
Objective:To analyze the fragmentation patterns of spirostanol steroidal saponins with UPLC-MS/MS in Anemarrhena asphodeloides Bge,Ophiopogon japonicus and Dioscorea opposita Thunb.Methods:Five kinds of spirostanol steroidal saponins were investigated with ESI + under low,middle and high collision energy.Results:The characteristic fragmentation patterns of five spirostanol steroidal saponins were summarized in positive mode,it was found that the spirostanol steroidal saponins firstly lost C-3 sugar groups,then characteristic aglycone ions were obtained by the cleavage of the E-ring and H2O.Conclusion:Characteristic fragmentation patterns of the spirostanol steroidal saponins could be significant to analyze other steroidal saponins.关键词
螺甾烷醇型甾体皂苷/超高效液相色谱-串联质谱/裂解行为Key words
Spirostanol steroidal saponins/UPLC-MS/MS/Fragmentation pattern分类
医药卫生引用本文复制引用
夏永刚,宫凤秋,郭信东,梁军,匡海学..知母、麦冬和薯蓣中螺甾体皂苷的质谱裂解规律研究[J].中医药学报,2017,45(5):10-13,4.基金项目
全国百篇优秀博士学位论文作者专项资金(201367) （201367）
黑龙江省杰出青年科学基金(JC2016020) （JC2016020）
黑龙江省普通本科高等学校青年创新人才培养计划(2016209) （2016209）
教育部重点实验室—北药基础与应用研究开放课题基金资助(2015bs06) （2015bs06）
哈尔滨市应用技术研究与开发项目(2017RAXXJ059) （2017RAXXJ059）
